Western Blotting - FAQS.TIPS

Shrikant Patil @Shrikant_Patil19

08 August 2017 1 10K Report

Why must

1. electrophoresis of the protein sample, and

2. transfer to nitrocellulose

precede the actual probing of the sample with an antibody?

Timmy Le

Electrophoresis must occur to seperate the protein while the transfer to the membrane allows the antibodies to properly attach to the protein that you are interested in.

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