What is the need for serial dilution of the sample when performing an Elisa?
You need to do serial dilution of the standard sample to make the standard curve in ELISA, and then again for the unknown samples in ELISA.
Antibody – antigen interactions might involve all types of chemical bonding, except which one? Explain.
07 August 2017 2,293 5 View
Why must 1. electrophoresis of the protein sample, and 2. transfer to nitrocellulose precede the actual probing of the sample with an antibody?
07 August 2017 9,508 1 View
Explain the statement “All immunogens are antigens, but not all antigens are imunogens.”
07 August 2017 4,712 7 View
Why Immunohistochemistry requires a conjugated enzyme, whereas immunofluorescence does not. Explain the difference?
07 August 2017 945 5 View
An investigator wanted to produce rabbit antiserum specific for mouse IgG, so she injected a rabbit with purified mouse IgG. However, to her dismay, the antiserum reacted with all isotypes of...
07 August 2017 8,415 7 View
I am planning to run an ELISA soon but I have a question regarding sample preparation. If I don’t know the working range of my samples for the ELISA, should I just add the samples to the plate...
07 August 2017 5,462 4 View
What are some common treatments that could be used to disrupt the antibody-antigen complexes?
07 August 2017 5,565 4 View
What is the function of the enzyme bound to the secondary antibody?
07 August 2017 8,198 9 View
What are the differences and similarities between western blotting and immunohistology?
07 August 2017 2,178 3 View
The indirect method requires use of a ‘secondary’ antibody, whereas the direct methodology does not. Explain the difference.
06 July 2017 3,047 4 View
I am developing an ELISA kit for rotavirus A (G1P8 and G1P6). Does anyone know the weight (ng) of these two strains? Or to help me find their weight?
29 July 2024 3,138 2 View
hi, I am facing difficulties in the développement of an ELISA sandwich. I have several experiences in developing ELISA but that’s the first time I can’t find any solution. My test is currently as...
16 July 2024 9,044 10 View
I placed mice in metabolic cages to collect urine samples for analysis. However, the majority of the samples have dried up. Is there any way to retrieve the urine in those tubes without...
14 July 2024 6,724 0 View
Anyone have a protocol for making cell lysate from rat cardiomyocyte pellets to run an ELISA using PBS as the buffer?
23 June 2024 2,264 4 View
If cells obtained from different passages, with different plating but harvested on the same day are used in a single plate to take an ELISA measurement (with the given standards and treated...
17 June 2024 6,705 1 View
I used two standards (one came with the ELISA kit and the other I made myself, by preparing a series of known concentrations of the target analyte) on the same plate in my ELISA run. Why are the...
15 June 2024 5,109 1 View
Dear fellow researchers, We are conducting an ELISA with the "Cattle IGF 1 (Insulin-like growth factor) ELISA Kit" (Cat: ELK5630). Samples and reagents were prepared following the instruction...
13 June 2024 9,473 0 View
We have to report the concentration in pg/ml, the ELISA kit provider did not reveal the specific activity of the enzyme. In that case how to convert the units?
05 June 2024 2,173 5 View
I have a question about AUC for calculating SARS-CoV-2 neutralizing antibody tests. AUC here is not the Area Under the ROC Curve. After six serial dilutions of human serum, an indirect ELISA was...
05 June 2024 8,191 0 View
I want to measure a protein in serum using an ELISA kit. This kit allows serum samples to be diluted 200 to 3200 times, but I want to use this kit to titrate higher concentrations of protein. Can...
03 June 2024 4,937 0 View