18 Questions 41 Answers 0 Followers
Questions related from Shrikant Patil
Why must 1. electrophoresis of the protein sample, and 2. transfer to nitrocellulose precede the actual probing of the sample with an antibody?
08 August 2017 9,414 1 View
What are the differences and similarities between western blotting and immunohistology?
08 August 2017 2,071 3 View
An investigator wanted to produce rabbit antiserum specific for mouse IgG, so she injected a rabbit with purified mouse IgG. However, to her dismay, the antiserum reacted with all isotypes of...
08 August 2017 8,375 7 View
What are some common treatments that could be used to disrupt the antibody-antigen complexes?
08 August 2017 5,507 4 View
I am planning to run an ELISA soon but I have a question regarding sample preparation. If I don’t know the working range of my samples for the ELISA, should I just add the samples to the plate...
08 August 2017 5,424 4 View
Why Immunohistochemistry requires a conjugated enzyme, whereas immunofluorescence does not. Explain the difference?
08 August 2017 885 5 View
What is the function of the enzyme bound to the secondary antibody?
08 August 2017 8,160 9 View
What is the need for serial dilution of the sample when performing an Elisa?
08 August 2017 1,648 1 View
Antibody – antigen interactions might involve all types of chemical bonding, except which one? Explain.
08 August 2017 2,249 5 View
Explain the statement “All immunogens are antigens, but not all antigens are imunogens.”
08 August 2017 4,670 7 View
The indirect method requires use of a ‘secondary’ antibody, whereas the direct methodology does not. Explain the difference.
07 July 2017 3,003 4 View
Which techniques are most appropriate for quantifying an antigen in a sample?
07 July 2017 403 3 View
What peptide sequences for immunisation will suitable? How to find out?
07 July 2017 3,984 4 View
I am interested in searching best possible conjugate to make melatonin as suitable immunogen. Any full-paper research or procedure if it is available then please share with me.
07 July 2017 288 2 View
The difference between chronic hypoxia and acute hypoxia is that in the latter case the cells return to the normoxic state. Acute and chronic hypoxia might lead to different biology within the...
07 July 2017 2,483 2 View
Which Phase of cell cycle is most radiation sensitive.
07 July 2017 2,599 1 View
I am going to perform cell viability assay after treatment with inhibitors. Which one would you advice me to use? Which viability test is better ? MTT or trypan blue ?
07 July 2017 8,213 14 View
Which techniques are most appropriate for quantifying an antibody in a sample?
07 July 2017 7,620 3 View
