I am doing an electroporation of Plasmid DNA (~1kbp) and electrocompetent E. coli DH5a. How many uL of cells and DNA should I use. I have 1uL DNA and 25uL cells per NEB protocol but I have found varying answers.
As Michael Swyers wrote, just use 1 uL DNA if all you need is transformed bacteria (and no quantification of efficiency etc). Depending on the competency of the cells and the DNA used, you (most likely) need to spread dilutions of your transformation to get well-separated cfus.