Electroporation of Plasmid DNA and electrocompetent E. coli DH5a, how much to use of each?

I am doing an electroporation of Plasmid DNA (~1kbp) and electrocompetent E. coli DH5a. How many uL of cells and DNA should I use. I have 1uL DNA and 25uL cells per NEB protocol but I have found...

15 November 2017 7,149 4 View