Electroporation of cripr cas9 by gene pulser?

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How to culture adipose stem cells and bone marrow stem cells cell lines ?

Kindly , I have human ASCs and BM-MSCs cell lines from ATCC. They mentioned on the website that they are cultured on mesenchymal stem cells media ! However, I have on my lab DMEM low glucose...

10 November 2018 8,966 4 View

Suitable insulin for cell culture ?

I am confused about the difference between the three components and which is suitable for cell culture as I bought the first one and it is not written if suitable for cell culture or not...

08 September 2018 8,143 0 View

Neuroblastoma SH-Sy5 and DMEM Mega cell medium ?

Kindly I need to coculture between neuroblastoma cell line (which I culture in DMEM low glucose medium with 10 % FBS) and other cell line that needs to be cultured in DMEM Megacell medium , So I...

07 August 2018 4,996 0 View

Which is better Qiagen mini prep Plasmid purification kit or promega ?

I am doing plasmid purification for about 20 Microgram yield , I wonder which is best Qiagen mini prep or promega ? and is the expiry date of Qiagen kit 1 year from opening the kit or the...

03 April 2018 546 3 View

Image J analysis?

How can I set ascale bar using image j soft ware for already taken images with leica inverted microscope. I have already followed the following steps: 1) Open the image using image j 2)From...

03 April 2018 6,183 0 View

Best Gel documentation system ?

Please, I don't know which is better Bio rad Chemi DOC xrs+ or Azure Biosystems™ c280 for protein and DNA gel applications !

03 April 2018 3,548 5 View

Does any body know whether MicroPulser™ Electroporator works for transfection of mammalian cells ?

I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...

02 March 2018 3,202 4 View

Size of electroporator cuvette size for mammalian transfection ?

I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !

02 March 2018 2,079 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

AMAXA Lonza nucleofection lower volume of buffer?

Does anyone tried to do nucleofection with AMAXA by Lonza with lower than recommended amount of buffer in the cuvettes (100 ul)?

07 August 2024 4,616 0 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View