How to culture adipose stem cells and bone marrow stem cells cell lines ?

Kindly , I have human ASCs and BM-MSCs cell lines from ATCC. They mentioned on the website that they are cultured on mesenchymal stem cells media ! However, I have on my lab DMEM low glucose...

10 November 2018 8,909 4 View

Suitable insulin for cell culture ?

I am confused about the difference between the three components and which is suitable for cell culture as I bought the first one and it is not written if suitable for cell culture or not...

08 September 2018 8,094 0 View

Neuroblastoma SH-Sy5 and DMEM Mega cell medium ?

Kindly I need to coculture between neuroblastoma cell line (which I culture in DMEM low glucose medium with 10 % FBS) and other cell line that needs to be cultured in DMEM Megacell medium , So I...

07 August 2018 4,938 0 View

Which is better Qiagen mini prep Plasmid purification kit or promega ?

I am doing plasmid purification for about 20 Microgram yield , I wonder which is best Qiagen mini prep or promega ? and is the expiry date of Qiagen kit 1 year from opening the kit or the...

03 April 2018 487 3 View

Image J analysis?

How can I set ascale bar using image j soft ware for already taken images with leica inverted microscope. I have already followed the following steps: 1) Open the image using image j 2)From...

03 April 2018 6,132 0 View

Best Gel documentation system ?

03 April 2018 3,494 5 View

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I am trying to do transfection of SNU 449 hepatocellular carcinoma cells by GFP plasmids using Bio Rad micropulser electroporator ! according to its protocol it nables safe and reproducible...

02 March 2018 3,141 4 View

Size of electroporator cuvette size for mammalian transfection ?

I am trying to transfect SNU449 cell line by GFP plasmids using genepulserX Bio rad electroporator. I wonder if I can use .4 cm cuvette and voltage needed ! and what is the cell size of SNU449 !

02 March 2018 2,027 4 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View