How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

Recommend antibodies that works in paraffin sections for synaptic and astrocytes markers in rat samples?

Hello, I am trying to find antibodies that can be used in paraffin rat brain sections against S100B , PSD95, Synapsin-I, VGLUT1,VGLUT2. I am checking the technical info provided by some companies...

12 August 2020 5,382 2 View

Innovation, Ideas permeability. Which factors make an idea to be commercialized?

There are a lot of new ideas in different fields, but only few will be innovative (new+comercialiced). As an example, Escalators and moving walks have the same mechanical design from more than...

13 July 2020 7,807 12 View

What is the minimum viral load that can be detected by TEM or SEM?

Imagine SARS-COV-2 in a surface to be recovered with an hyssop .

08 July 2020 1,193 3 View

Any good manual for phenomenological approach to sustainability?

I'm thinking about a turn from my ethnographic approach to a phenomenological approach in the study of sustainability and forest preservation. However, I didn't find any good manual to design and...

06 July 2020 9,618 1 View

Are there someone thinking about a simulation software for evacuation/circulation tasks including COVID-19 propagation?

I think it would be interesting to implement the propagation of the COVID-19 virus in some simulation software for the circulation of people, for a population diverse in ages, heights, etc.,...

30 June 2020 7,481 14 View

COVID-19, Lessons learned ?

It should be mandatory to learn from what life teaches us. With COVID-19, we have been forced to buy sanitary materials because in our country there was no industry to do so (dependency) and we...

30 June 2020 3,018 36 View

How to extract polygons of species distribution from books?

If you need a distribution map from a book, how do people deal with that? Do u email authors to obtain the layers or is there a way to export the polygons from the book images? Colleagues and I...

20 June 2020 4,391 1 View

Can someone recommend papers regarding time course lignin accumulation?

I'm looking for information regarding the dinamic of lignin accumulation during in vitro culture of plants.

16 April 2020 3,699 5 View

Is it better to use base e (LN) or base 10 (LOG10) when making estimates of body mass from linear measurements?

For linear regressions between linear measurements and body mass, which transformation is better to use? base e logarithm (LN)? or base 10 logaritm (LOG10). Apparently they do not make such a for...

08 April 2020 5,201 12 View

Can linear DNA be used as template for site-directed mutagenesis?

Is it possible to induce site-directed substitution mutation by quick-change method on linear dsDNA? or it has to be cloned in some vector? If yes, should it be treated with the Dpn1 enzyme...

03 March 2021 401 4 View

No title

02 March 2021 3,060 3 View

Does anyone have a recommendation for yeast reporter gene plasmid/protocol?

Hi, could anyone recommend a plasmid and/or protocol for reporter gene assay in S. cerevisiae? I want to assess the effect of growth conditions on a transcription factor, so I want to clone it`s...

01 March 2021 210 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can someone suggest a way to prepare slides to observe under confocal microscope for observing fluorescent tagged proteins??

I am growing the cells on coverslips and transfecting the fluorescent tagged protein directly on them. Then i want to observe these cells under confocal microscope. Currently i am just using...

01 March 2021 6,142 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View