Electroporation conditions for primary retinal cell cultures (chick embryo)?

Carlos Guillermo Martinez-Moreno @Carlos_Martinez-Moreno

08 August 2016 0 10K Report

We are trying to transfect primary retinal cell cultures (chicken) with a pCAG (Addgene) plasmid but we have very low transfection efficiency (

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What is the status of segmented labor markets theory today? How it can be tested empirically?

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What measures can be done to reduce the increase in LDH activity in serum and heparin plasma samples due to underfilled vacuum blood collection tubes?

This question is written in relation to the article written by Neuwinger, Nick et al. entitled “Underfilling of vacuum blood collection tubes leads to increased lactate dehydrogenase activity in...

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How does the Sysmex DI-60 digital morphology analyzer help evaluate the shape of blood cells, and what insights does it provide on time for analysis?

This question is asked in relation to the article, "Red and white blood cell morphology characterization and hands-on time analysis by the digital cell imaging analyzer DI-60". The mentioned study...

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What are the laser requirements for a Brillouin microscope?

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Posthoc 2 Factors RStudio?

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How do I build an Intercorrelation Matrix with 20 financial ratios (FRs) of 10 firms over the past five years using SPSS?

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Sauropod-like vertebrate teeth?

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Does anyone know how to remove the chromogen from an immunohistochemistry?

During my immunohistochemistry procedure, after applying the chromogen diaminobenzidine, I mistakenly added other reagents—PBS, Triton, avidin, and biotin—resulting in chromogen precipitation and...

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Do code schools / coding bootcamps have a positive impact on local or regional hiring in technology related fields?

I'm looking to explore the relationship between coding schools in a community / region, and regional economic development in technology related fields. Are companies in a community or region who...

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Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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How to confirm the site-directed mutagenesis result without performing NGS?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

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Why activated CAR-Jurkat cell could not kill targets?

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Why does my protein refolded to beta sheet during thermal denaturation analysis?

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

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Does long-term culture of murine Monocytes result in macrophage differentiation?

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