Does anyone know how to remove the chromogen from an immunohistochemistry?

01 December 2023 1 7K Report

During my immunohistochemistry procedure, after applying the chromogen diaminobenzidine, I mistakenly added other reagents—PBS, Triton, avidin, and biotin—resulting in chromogen precipitation and the formation of dots across the tissue. Does anyone know how to remove the chromogen or fix the tissue?

Ellen A G Chernoff

This works for well-attached sections. Article Antibody Elution Method for Multiple Immunohistochemistry on...

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