Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Which sampling techniques can be used for huge flying insects?

Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...

13 January 2021 3,735 5 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View

How is vertical agriculture progressing in Colombia?

Vertical agriculture offers an innovative solution to reduce the pressure of cultivation on soils. The intensive use of machinery and large areas of land harm biodiversity.

28 October 2020 8,773 3 View

Why do some primers have transcript variants/unintended templates for different genes?

Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...

05 October 2020 352 3 View

Do I need to design forward and reverse primers within an exon region for QRT-PCR?

Good day, I hope you are all well. I am new in designing primers for quantitative reverse transcription PCR. I am using NCBI to design my primers and my question is: Do I have to design my...

04 October 2020 7,678 7 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

How seminal was the influence of Giuseppe Campos Venuti and Federico Oliva in Italy, France, Spain and other countries?

The question is what remains in the urban planning and in our professional and intellectual experiences of Giuseppe Campos Venuti and Federico Oliva? What about discipline now? They reasoned and...

30 October 2020 5,450 4 View

Does anyone recommend an antibody for cAMP detection in mouse brain tissue (WB or IHC)?

21 April 2020 6,336 0 View

How to extract cAMP from mouse liver?

Dear all, I would like to ask the method to extract cAMP from mouse liver. I found some papers homogenizing the tissue in HCl. Is this the most appropriate method? Thanks

12 March 2020 516 1 View

Help, my agarose is dissolving in the tissue processor?

Dear, I am trying to use a 1% agarose gel to replace the current in use HistoGel from Richard-Allan scientific. The HistoGel has tend to dissolve when it goes thru the tissue processor...

06 July 2019 7,007 3 View

How to evaluate the error between process simulation result and read data?

We are tackling exploiting a process simulator that receives some initial input data and outputs time series transition in parameters, and we are comparing these results and real data (using as...

24 June 2019 4,544 5 View

No title

05 June 2019 5,483 2 View

How to evaluate the error between process simulation result and read data?

We are tackling exploiting a process simulator that receives some initial input data and outputs time series transition in parameters, and we are comparing these results and real data (using as...

05 June 2019 2,441 6 View

-O ser humano tem capacidade de descriminar a DOR em PONTADA?

10 November 2018 565 2 View

No title

10 November 2018 4,723 1 View

No title

09 October 2018 6,558 2 View