I did apply the Linda Chalker Scott method that is on her thesis. It is relevant to use the releases at ambient and overlethal temperatures as relative parameters for scaling. Leaf age and water salinity change these reference values, and several authors apply other releases as phenolics. http://ir.library.oregonstate.edu/xmlui/bitstream/handle/1957/25663/CHALKERSCOTTLINDA1989.pdf?sequence=1#page=74

Dionisio-Sese ML, Tobita S. 1998. Antioxidant responses of rice seedlings to salinity stress. Plant Sci. 135, 1-9.

Thank you friends

In cold stressed coffee plants, we followed the protocol described in this paper: 

https://www.researchgate.net/publication/10758139_Electrolyte_leakage_and_lipid_degradation_account_for_cold_sensitivity_in_leaves_of_Coffea_sp_plants

Article Electrolyte leakage and lipid degradation account for cold s...

 Thanks Paula

In heat stressed tomato plants, i used the protocol described in the thesis here:

see Page 10

http://utdr.utoledo.edu/cgi/viewcontent.cgi?article=1103&context=theses-dissertations

you can see my publication on cold stress and electrolyte leakage

Article Exogenous application of plant growth regulators (PGRs) indu...