EDC-NHS coupling? - FAQS.TIPS

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How to remove magnetic beads from exosomes?

I am isolating exosomes by magnetic beads coupled to antibodies. Now, I need to remove magnetic beads from my exosomes! what protocol do you suggest? thanks

20 December 2022 9,004 2 View

How to solve the problem of omitting ASC because of no within-group variance in STATA?

Dear All, I conducted a choice experiment to investigate consumer behavior towards corn oil; then, I analyzed my data by using stata for " Conditional Logit Model". In its result there is no...

30 April 2022 7,824 0 View

Which nudge do you recommend to affect consumer purchase intention in a hypothetical choice experiment?

I want to conduct a hypothetical choice experiment to investigate the impact of nudges on consumer purchase intention towards GMOs. The informative nudge is the one that affects consumers' slow...

26 February 2022 6,916 0 View

I have to use the Midlife Development Inventory personality test in my questionnaire. the target population is Persian. can I translate it on my own?

I want to publish a paper on consumer behavior based on this survey. So I want to be sure that there won't be any problems because of my translation. My English level is okay, but I thought maybe...

13 February 2022 1,764 5 View

Python code for U-NET for MRI and CT segmentation?

Dear colleagues; I am looking for a Python code to segment the lesions on CT and MRI images using UNET. I need a generic code which, by changing a few words, I can reuse for different lesions. The...

13 December 2021 3,420 3 View

Brain MR image Normalization before Radiomics feature extraction in 3D slicer?

Hello; Anybody knows how to perform normalization of brain MR images before feature extraction (Radiomics) in "3D slicer"? Also what is the best filter for brain MRI before radiomics. Thank you...

02 December 2020 3,326 1 View

Convolutional Neural network for stack of CT or MRI images?

Hello; I know how to train image classifiers (CNN) for classification of single cross-sectional CT or MRI image. However, I don't know how to send all images from one patient to the model. I need...

10 September 2020 9,539 4 View

Anybody interested in conduction a clinical trial about hemoglobin F in COVID-19?

Dear Colleagues; We observed less mortality of COVID-19 in regions with high hemoglobinopathy rates. This does not prove the effect of hemoglobin on this infection but it may worth to test it....

17 June 2020 9,403 4 View

How to plot a beam?

There is a uniform linear array with N transmitters. Let matrix S denotes transmitted signals. In fact S is an N*L matrix where N is the number of transmitters and L is the samples(snapshots) of...

29 May 2020 9,955 2 View

How Signal Power in MIMO Radar can be calculated?

Power of a transmitted radar signal, s[n] can easily be calculated as : P=(1/N) s[n] ' * s[n] where s[n] is an N*1 vector and ' denotes hermition. But how about power calculation for a MIMO...

13 April 2020 3,767 1 View

Is the mentioned CV graph a valid one as this graph have only one peak prominent (reduction)?

I have used Prussian blue nanoparticles as a redox couple. The PBNPs have been made using only one salt precursor. Also, during scan rate studies, a small oxidation peak can be consistently found...

31 July 2024 9,697 0 View

How to draw photon-magnon coupling color plot in origin?

Please help me in plotting the photon-magnon coupling plot as shown below

14 July 2024 1,632 1 View

How to solve the question of ansys fluent?

Regarding the system coupling issue where the update of the solution component fails, the system coupling update for fluid flow (Fluent) triggered an exception: "Update Solution Failed."

30 June 2024 7,894 1 View

Does anyone have suggestions for this esterification?

I have a very polar alcohol (soluble in MeOH, H2O, partially in heated DMSO). And a long chain fatty acid that I would like to couple. I can't find a common solvent for them. I can form the...

24 June 2024 9,319 0 View

How to verify the correctness of an abaqus model and how to make a thermally coupled model converge to the correct values?

Hi all, I am doing a hot rolled composite simulation, but the same model, using the implicit and explicit algorithms gives the results shown below, why do the two algorithms give completely...

14 June 2024 7,737 1 View

How to FE model from Modal analysis to use in Static Structural in Ansys?

I have two components for which I want to extract some number of modes shapes for each. Then using those model of the component I want to couple them together for non linear static analysis.

10 June 2024 1,234 0 View

How do couples enjoy adventurous sex play?

Most couples who rate their sex lives highly say they are proactive in sex. They think about when, where and how they’ll have it and think up new things to try. (Tracey Cox)

05 June 2024 6,504 5 View

How can sex therapists ignore the concept of sexual consent?

Men want more sex than they usually get. … It’s no wonder, then, that couples argue most often about sexual frequency… (Kramer & Dunaway)

02 June 2024 1,238 2 View

Is it easier to couple polypeptides after a few days of dissolving them in PBS?

I want to couple the polypeptide to a cell culture plate, is it easier to couple the polypeptide after a few days of dissolution in PBS?

21 May 2024 9,684 1 View

For immunofluorescence of mouse embryos, is there a best way to store semi-long term?

I'm on a time crunch and have to fly back home and my return date to the lab where I'm doing my research is yet to be determined (hopefully in the next couple of months). I'm hoping I can store at...

15 May 2024 2,572 0 View

Houman Kholafazad Kordasht

Michael Uebel Thanks

Daru Seto Bagus Anugrah

This is a usefull link:

https://www.thermofisher.com/kr/ko/home/life-science/protein-biology/protein-biology-learning-center/protein-biology-resource-library/pierce-protein-methods/carbodiimide-crosslinker-chemistry.html

Daru Seto Bagus Anugrah Thank you so much for your kind response.

Kumara B N

Dear Houman,

This may helpful for you.

https://info.gbiosciences.com/blog/2-step-protein-coupling-edc-nhs

M. A. Ahmed

After this coupling, How can i broke it?

Kumara B N Thank you for your kind responsive.

M. A. Ahmed It wasn't my aim. Therefore, I have any idea about this.