I am isolating exosomes by magnetic beads coupled to antibodies. Now, I need to remove magnetic beads from my exosomes! what protocol do you suggest?
thanks
I suggest you read the title "Isolation of Exosomes from Exosome-Bead Complexes" in the link. (https://patents.google.com/patent/WO2018172384A1/en)
Best regards.
If the exosomes do tolerate it, try a reducing agent like DTT or preferably TCEP to dissociate the antibodies into fragments by breaking the disulfide bridges.
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