Easy method for Inclusion bodies quantification?

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Adam B Shapiro

Inclusion bodies can be dissolved in guanidine HCl for subsequent purification and refolding. Here is a guide to handling inclusion bodies:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3518028/

One of the common protein assays can be used to measure total protein content of the guanidine-dissolved inclusion bodies. Here is a chart showing the tolerances for guanidine of the various protein assays.

Steingrimur Stefansson

Hi Nadia, if you have to measure inclusion bodies for proteins expressed in E coli then you have real problems.

Nadia Samak

Thanks a lot for your answers