What is the unit of intracellular Reactive Oxygen Species (ROS)?

10 October 2016 0 5K Report

I measured intracellular ROS for bacterial cells so what will be the unit ? i can say Micro mol/g or what? I'll appreciate your reply.

Badges
Science topic

Similar topics
Psychometrics
Measurement

More Nadia Samak's questions See All

Hi, Can any one give me equation for measuring the relative and residual activity of an enzyme?

I need to measure the relative and residual activities for my enzyme is there any simple equation to understand how to measure them?

31 December 2016 9,724 5 View

Easy method for Inclusion bodies quantification?

I need to know an easy and simple method to quantify inclusion bodies produced from E. coli for protein expression

08 September 2016 9,077 3 View

Why different PCR band appear in gel electrophorysis?

I did cloning for certain insert (1155 bp) into vector (5000 bp) and after transformation i checked the success of transformation by doing PCR for resulted colonies and I got band in the right...

06 July 2016 6,618 9 View

I have DNA template which have 2 restriction enzyme sites how can I change these restriction sites?

I have DNA template which have 2 restriction enzyme sites but I wanna change these restriction sites to another restriction enzymes, can I design new primers with the new restriction sites I want...

05 June 2016 5,091 5 View

Which reference gene primer can be used to normalize qRT-PCR data from E. Coli?

I want to study mRNA expression of my gene at E. coli (I did cloning for the gene from bacillus to E. Coli before), I finished the extraction step for RNA but I don't know which reference gene...

02 March 2016 8,068 4 View

I wanna ask about the difference between relative activity and specific activity?

I also wanna know how to calculate them for laccase i usually calculate laccae activity as U/L.I have attached article for more explanation.

11 December 2015 627 1 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Aspen Plus Simulation?

Greetings When one is to perform Simulation or modelling of Pyrolysis of Biomass Waste materials using Aspen Plus software, and one has both the macromolecules breakdown (Cellulose, Lignin and...

06 August 2024 5,023 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

Measuring the Intelligence of a Species?

Larger brains, which typically contain more neurons, store and transfer more information (Tehovnik and Chen 2015), but the precise relationship between number of neurons and information has yet to...

05 August 2024 1,238 2 View

How does one derive the standard deviation of a scale?

Dear all, I am working on analyzing data from a survey on student satisfaction. The survey contains items with a 7-point Likert response format that produce 12 scales related to different areas...

05 August 2024 2,141 4 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Why results of ROS flurescence are negative as there was no bacteria within?

Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...

04 August 2024 5,974 3 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Why aren't most metal oxides insoluble in water?

Why BaO (s) + H2O(l) ---> Ba 2+(aq) + 2 OH - (aq) if Oxygen loves owning all the electrons, why would it bind to hydrogen which competes with Oxygen for electron ownership more so than...

03 August 2024 5,392 1 View