I measured intracellular ROS for bacterial cells so what will be the unit ? i can say Micro mol/g or what? I'll appreciate your reply.
I need to measure the relative and residual activities for my enzyme is there any simple equation to understand how to measure them?
31 December 2016 9,634 5 View
I need to know an easy and simple method to quantify inclusion bodies produced from E. coli for protein expression
08 September 2016 8,950 3 View
I did cloning for certain insert (1155 bp) into vector (5000 bp) and after transformation i checked the success of transformation by doing PCR for resulted colonies and I got band in the right...
06 July 2016 6,515 9 View
05 June 2016 5,013 5 View
I want to study mRNA expression of my gene at E. coli (I did cloning for the gene from bacillus to E. Coli before), I finished the extraction step for RNA but I don't know which reference gene...
02 March 2016 7,970 4 View
I also wanna know how to calculate them for laccase i usually calculate laccae activity as U/L.I have attached article for more explanation.
11 December 2015 522 1 View
Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...
03 March 2021 5,999 3 View
I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...
02 March 2021 9,945 3 View
im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...
02 March 2021 2,246 2 View
Is DCFDA appropriate for measuring total ROS production, or can it only detect certain types of ROS? If I want to measure total ROS production, which dye should I use?
01 March 2021 9,447 3 View
When you use RIA, with a control the unknown sample with antibodies, you bare in mind the binding sites of the antibodies. However you still need to measure labelled antigen (radioactive) and not...
28 February 2021 2,133 3 View
I like to do project in laser measurement system. My objective is to eliminate or reduce the cosine error, abbe error in Laser measurement system. I have Rhenishaw XL80 system. Please tell the...
28 February 2021 4,890 3 View
28 February 2021 2,534 2 View
based on what I've studied on multiple papers, for peptide and protein identification Alpha-cyano-4-hydroxycinnamic acid (CHCA) is an appropriate matrix to use, I also know that the CHCA powder...
26 February 2021 6,799 3 View
I diluted siRNA and RNAiMAX in opti-MEM and added to the cells which they were in the growth medium. Is it a right way? or should I culture cells in the opti-MEM medium for a while and not in...
26 February 2021 10,041 3 View
I am looking to package lentivirus in endothelial cells but I am opposed to using 293 cells due to personal ethics regarding the source. I have done some reading and saw that COS1, COS7, & CHO...
25 February 2021 3,030 1 View