Nadia Samak

7 Questions 11 Answers 0 Followers

Questions related from Nadia Samak

Hi, Can any one give me equation for measuring the relative and residual activity of an enzyme?

I need to measure the relative and residual activities for my enzyme is there any simple equation to understand how to measure them?

01 January 2017 9,698 5 View

What is the unit of intracellular Reactive Oxygen Species (ROS)?

I measured intracellular ROS for bacterial cells so what will be the unit ? i can say Micro mol/g or what? I'll appreciate your reply.

10 October 2016 5,336 0 View

Easy method for Inclusion bodies quantification?

I need to know an easy and simple method to quantify inclusion bodies produced from E. coli for protein expression

09 September 2016 9,057 3 View

Why different PCR band appear in gel electrophorysis?

I did cloning for certain insert (1155 bp) into vector (5000 bp) and after transformation i checked the success of transformation by doing PCR for resulted colonies and I got band in the right...

07 July 2016 6,584 9 View

I have DNA template which have 2 restriction enzyme sites how can I change these restriction sites?

I have DNA template which have 2 restriction enzyme sites but I wanna change these restriction sites to another restriction enzymes, can I design new primers with the new restriction sites I want...

06 June 2016 5,068 5 View

Which reference gene primer can be used to normalize qRT-PCR data from E. Coli?

I want to study mRNA expression of my gene at E. coli (I did cloning for the gene from bacillus to E. Coli before), I finished the extraction step for RNA but I don't know which reference gene...

03 March 2016 8,047 4 View

I wanna ask about the difference between relative activity and specific activity?

I also wanna know how to calculate them for laccase i usually calculate laccae activity as U/L.I have attached article for more explanation.

12 December 2015 600 1 View