Hi, Can any one give me equation for measuring the relative and residual activity of an enzyme?

I need to measure the relative and residual activities for my enzyme is there any simple equation to understand how to measure them?

31 December 2016 9,634 5 View

What is the unit of intracellular Reactive Oxygen Species (ROS)?

I measured intracellular ROS for bacterial cells so what will be the unit ? i can say Micro mol/g or what? I'll appreciate your reply.

09 October 2016 5,273 0 View

Easy method for Inclusion bodies quantification?

I need to know an easy and simple method to quantify inclusion bodies produced from E. coli for protein expression

08 September 2016 8,950 3 View

Why different PCR band appear in gel electrophorysis?

I did cloning for certain insert (1155 bp) into vector (5000 bp) and after transformation i checked the success of transformation by doing PCR for resulted colonies and I got band in the right...

06 July 2016 6,515 9 View

I have DNA template which have 2 restriction enzyme sites how can I change these restriction sites?

05 June 2016 5,013 5 View

I wanna ask about the difference between relative activity and specific activity?

I also wanna know how to calculate them for laccase i usually calculate laccae activity as U/L.I have attached article for more explanation.

11 December 2015 522 1 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Problem with shRNA transfection. Why are only two plates getting contaminated instead of all?

I transfected my LNCaP-WT cells with 3 shRNA plus their NTC two weeks ago and split two puromycin selected cell plates on Friday last week(Feb 26). I checked for GFP in the cells, and they all...

28 February 2021 4,949 3 View