Hi, I did heterologous expression of reverse transcriptase in different strains of E. coli at 3 temperatures, 37 °C, 28 °C and 20 °C. As I expected, BL21 provided the best solubility at 20 °C and the worst at 37 °C. On the other hand strain MC4100 had the best solubility at 37°C and the worst at 20 °C. Has anybody similar experience with E. coli MC4100?

Thanks for responses!