Fermentation affect activity of Reverse transcriptase?

More Bohuš Kubala's questions See All

ATG in pPICZalfphaA vector: Yes or Not in front of the gene of interest ... ?

Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...

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Is it possible to get gene from Boletus edulis which is more similar to Suillus sp. ?

Hi, I isolated genomes from different fungi. One of them is Boletus edulis (according ITS and 28srRNA primers ~99%). But sequencing of peroxidase gene, which was amplified by specific primers for...

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What SUPEROXID DISMUTASE primers do you use for identification of fungal species ?

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24 May 2023 2,570 2 View

Single primer PCR - any positive experiences ?

Hi, I would like to ask if anybody has positive experiences with single primer PCR ? Can you recommend me any proven protocol of this type of PCR ? Thank you for all recommendations. Bohuš

08 March 2023 4,641 3 View

What light is appropriate for in vitro plant cultures ?

Hi, I would like to ask what light do you recommend to use for in vitro plant cultures? I use full-spectrum LED light (it is pink shining). Is this appropriate for purpose of regeneration of plant...

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Low reverse transcriptase activity from fermenter?

Hi, is it possible, that fermentation Can cause low activity of produced Reverse transcriptase? My RT from Erlenmayer flask is more active. But RT from fermenter Was expressed from T5 system and...

18 April 2022 8,379 0 View

What characteristics belong to Micella formation of anionic alkylbenzene sulfonic acid calcium salt in different solvent?

Regarding the pesticide industry it is a very common and general application of calcium dodecylbenzene sulfonate’s (CaDDBS) usage in the composition of emulsifiable concentrates The calcium...

09 August 2020 3,276 1 View

Cultivation of mycorrhizal fungi on beech roots.

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Reverse transcription Hand-made buffer

Hi guys, Is there anybody who mix Hand-made Reverse-transcription buffers? Is it possible to use Mn ions instead of Mg for better activity? I would be very grateful for All advices. Thank you so...

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How much methanol should be added to the fermenter?

How much methanol should be added to the fermenter in which we cultivate Pichia pastoris for the purpose of heterologous enzyme production? We will add methanol continuously with the pump x...

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Is there a problem with my RNA pellet?

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Can I base on reverse DNA sequences to perform alignment, convert to amino acids and GenBank submission?

I have reverse sequences (AB1 format), can I base on reverse DNA sequences to perform nucleotide alignment, convert nucleotides to amino acids and deposit the sequence in GenBank database?

11 August 2024 5,136 1 View

Strugglling with m6A dot blot any suugesstion ?

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RNA Extraction Using Hot Borate Method No Longer Working?

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08 August 2024 9,881 2 View

I can't see the ssDNA band after performing asymmetric PCR. Is there any way to do this?

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08 August 2024 1,666 3 View

Does crude extraction using NaOH and Tris work well with Fungi?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

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E.coli contamination in human RNA seq data ?

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How much Yeast should we add to fermentation media?

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Nicolai S Panikov

It is not surprising. Gene expression depends on cultivation conditions. But for the very start, you have to express enzyme activity per cell mass unit. Then you can compare flask vs bioreactor

Edilberto FLORES Aguilar

El cambio de escala de hecho cambia el comportamiento celular a considerar en la optimizacion de un bioproceso.

Peter Kysel

Antifoam from fermentation sticking to reverse transcriptase?