Hi, I'd like to ask about cultivation mycorrhizal fungi which are special beech roots. What media should I use? How to grow in vitro beech ? Thanks for advices.
Bohuš
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
Hi, I isolated genomes from different fungi. One of them is Boletus edulis (according ITS and 28srRNA primers ~99%). But sequencing of peroxidase gene, which was amplified by specific primers for...
18 June 2023 8,810 0 View
Hi, I would like to ask what primers for superoxid dismutase do you use or do you know for identification of fungal species ? I used ITS and NL primers but I need to use more markers. I would like...
24 May 2023 2,571 2 View
Hi, I would like to ask if anybody has positive experiences with single primer PCR ? Can you recommend me any proven protocol of this type of PCR ? Thank you for all recommendations. Bohuš
08 March 2023 4,642 3 View
Hi, I would like to ask what light do you recommend to use for in vitro plant cultures? I use full-spectrum LED light (it is pink shining). Is this appropriate for purpose of regeneration of plant...
13 February 2023 6,559 2 View
Hi, I would like to ask, why my reverse transcriptase from fermenter is less active than reverse transcriptase produced from flasks? Thank you for responses Bohuš
24 April 2022 8,738 3 View
Hi, is it possible, that fermentation Can cause low activity of produced Reverse transcriptase? My RT from Erlenmayer flask is more active. But RT from fermenter Was expressed from T5 system and...
18 April 2022 8,380 0 View
Regarding the pesticide industry it is a very common and general application of calcium dodecylbenzene sulfonate’s (CaDDBS) usage in the composition of emulsifiable concentrates The calcium...
09 August 2020 3,276 1 View
Hi guys, Is there anybody who mix Hand-made Reverse-transcription buffers? Is it possible to use Mn ions instead of Mg for better activity? I would be very grateful for All advices. Thank you so...
01 January 1970 7,962 0 View
Hi, I have problem to sterilize freshwater plants (Anubias, Bucephalandra). I used chlorine salts and ethanol. I got contaminations. I think AgNO3 could works. Do you have any other protocols for...
01 January 1970 2,807 5 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,731 2 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 179 3 View
Please discuss someone told about fungus and allied others.
31 July 2024 3,372 3 View
Now i am working on AM fungi single strain. What are the most effective isolation techniques for obtaining pure cultures of arbuscular mycorrhizal (AM) fungi?? Is there are best approach...
26 July 2024 6,520 1 View
I don't have the anaerobic generators (Anaeropac) so I'd like to try the candle-jar tecnique for the cultive of lactic acid bacteria
15 July 2024 8,890 2 View
How can we measure their colony diameter?
12 July 2024 7,027 0 View
I am carrying out an experiment on Trichoderma and need to measure OD of the fungal growth after 7 days. Unlike bacteria, fungus clump together making It difficult to get OD for the fungal growth....
11 July 2024 6,250 1 View
We have been experiencing repeated what looks very much like fungal contaminations in several incubators (in the same lab). We have tried the following repeated times and yet the coating on all...
02 July 2024 6,374 2 View
What did the plantation economy in the South produced and all the crops cultivated in north India cannot cultivated in south India why?
26 June 2024 7,264 0 View
I would like to become self disciplined and work by myself without supervision. How do I achieve it when I am not interested at all in working without any push by others?
24 June 2024 4,529 5 View