Hi Bohuš Kubala

maybe you'll find interesting this paper:

Article Parallel Molecular Evolution of Catalases and Superoxide Dis...

all the best

fred

I can provide some general guidance on finding primers for SOD genes in fungal species:

1.Existing literature: Search scientific literature, particularly studies focused on fungal identification or molecular characterization of fungi. Look for studies that have used SOD genes as markers and check if they have reported specific primer sequences. Relevant articles may provide primer information or references to previous studies that have used SOD genes for fungal identification.

2.Primer databases: Explore online primer databases, such as NCBI Primer-BLAST, PrimerBank, or OligoArchitect, which provide a collection of validated primers for various genes and organisms. While these databases may not specifically list SOD primers for fungi, they may contain primers designed for related genes or conserved regions that could potentially be adapted for your research.

3.Sequence alignment and primer design: If specific SOD primers for fungi are not readily available, you can consider designing your own primers based on conserved regions of the SOD gene across different fungal species. Obtain SOD gene sequences from different fungal species, align them using bioinformatics tools (e.g., Clustal Omega or MAFFT), and identify conserved regions suitable for primer design. Utilize primer design software like Primer3 or NCBI Primer-BLAST to design primers targeting these conserved regions.

Remember, primer design for gene amplification requires careful consideration of factors such as primer specificity, melting temperature, and primer length. It is recommended to validate the primers experimentally and optimize the PCR conditions to ensure reliable and specific amplification of the target SOD gene in the fungal species of interest.

And i can provide you with some general information on superoxide dismutase (SOD) primers that have been used in fungal identification studies.

The choice of SOD primers for fungal identification may vary depending on the specific fungal species or groups of interest. Here are a few examples of SOD primers that have been used in previous studies:

SOD1 primers:

Forward primer: 5'-ATGGTCTTGGTTGGACAGGC-3'

Reverse primer: 5'-TTAGCGGAGCTGTCCTGAGG-3'

SOD2 primers:

Forward primer: 5'-ATGTCAGCTTGCCTGATGG-3'

Reverse primer: 5'-GCTGAGGTTGACCGTTGTTG-3'

SOD3 primers:

Forward primer: 5'-ATGCTGGACTGCAGTGGCGT-3'

Reverse primer: 5'-GGATGGCTGGAAGTCGGCTG-3'

It's important to note that the above primer sequences are provided as examples and may not be suitable for all fungal species. The selection of primers should be based on the specific SOD gene isoform, the fungal species of interest, and the conservation of SOD gene sequences among different fungi.

Additionally, it may be necessary to design species-specific primers or adapt primers based on conserved regions of SOD genes among related fungal species. Bioinformatics tools and primer design software can assist in the design process by identifying conserved regions suitable for primer design.

Remember to validate the primers experimentally and optimize the PCR conditions to ensure reliable and specific amplification of the target SOD gene in your fungal species of interest.