The thing is we don't have chemidoc for the documentation of the blots. So previously we used Alkaline phosphatase-conjugated secondary antibodies and after incubation, we developed the blots by directly adding BCIP/NBT substrate on the membrane of the blots. (CALORIMETRIC BLOT) the bands will appear.
If I use the HRP-conjugated secondary antibody can I develop the blots by using a TMB/ABTS substrate on the membrane? (not the chemiluminescent substrate)
Kindly advise for the same.
Hello Deepdarshan Urs
If I use the HRP-conjugated secondary antibody, can I develop the blots by using a TMB/ABTS substrate on the membrane? (not the chemiluminescent substrate)
Yes, you can. See Pg 2 (Fig 2) of the link attached below.
https://www.jacksonimmuno.com/secondary-antibody-resource/immuno-techniques/chromogenic-detection-for-western-blot-ihc-and-elisa/?pdf=129
Best.