Deepdarshan Urs

5 Questions 28 Answers 0 Followers

Questions related from Deepdarshan Urs

During the westernblot the HRP Conjuagted secondary antibody is compatibel with TMB/ABTS substrate (not the chemiluminescent substrate)?

The thing is we don't have chemidoc for the documentation of the blots. So previously we used Alkaline phosphatase-conjugated secondary antibodies and after incubation, we developed the blots by...

18 September 2024 1,565 1 View

How long animal tissues can be stored in -20degree?

Dear All, Could you please share your expertise? We now have a lack of -80 freezer resources, so we need to keep items at -20 degrees Celsius instead.

13 August 2024 4,506 2 View

How can I determine the IC50 drug concentration to normal lung cells?

Dear All, I need to standardize synthesized drug concentration (IC50) for my in vitro experiment but I can't adopt MTT, LDH, or trypan blue kind of assay because I'm working on normal...

21 February 2024 988 3 View

What are the symptoms of Intransal inhalation of organic dust in the mice?

I'm inducing (Repeated exposure) lung inflammation by using organic dust; I want to know how we can determine whether the mice's lungs will inflammated or not. What are the symptoms clinical...

09 January 2023 2,200 2 View

What are some software/tools for LC50/ LC90 determination using the probit or trimmed Spearman-Karber method?

What are some software/tools for LC50 determination using the probit or trimmed Spearman-Karber method?

18 November 2021 8,536 3 View