For insoluble particles inflammation will depend on the concentration of inhaled particles. Rats are by far the most sensitive species to the effects of 'lung overload' following inhalation. When the lung burden exceeds about 1 mg/g in lung tissue, significant inflammation, epithelial hyperplasia, ... will be observed.

Hi Deepdarshan Urs

Have you done characterization of the organic dust? For VOC's, PAH'S or metals?

I expose C67/BL6 mice with PM 2.5 and the functional parameters of the mice lungs are affected from 15th day itself. So if you have instruments to assess the lung function such as whole body plethysmography or FlexiVent analysis, you could check the same at 15th day interval and from then on every 7 days interval to check how the inflammation and lung function are being affected due to repeated exposure of organic dust particles.

Thanks

Samir