DU145 cell(prostate cancer cell) xenograft? - FAQS.TIPS

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Z-average size from Dynamic Light scattering can it be lower than the original probe size?

Hi everyone, Recently I have been conducting Dynamic Light scattering experiments in a micellar solution at 5 and gel at 37 degrees of Celsius with latex particles of diameter 190-500 nm. While...

01 August 2024 1,168 4 View

Method for estimation of biomass using non-destructive method for ornamental plants?

Allometric equations are available for trees but couldn't find any method/equation for ornamental plants?

04 July 2024 2,646 1 View

How can CHARMM-GUI be used for protein-ligand interaction ?

My protein is a transmembrane protein, therefore i need to construct a transmembrane using CHARMM-GUI. Can someone please suggest me a tutorial for the same.

24 June 2024 5,044 1 View

Viscosity or Complex viscosity should be used in the SOP for Malvern Zetasizer or carrying out DLS experiments for polymer samples?

Hi all, I am trying to conduct Dynamic Light scattering experiments on polymer sample using Malvern Zetasizer ZS. One question I have is to whether it is okay to use complex viscosity instead of...

11 May 2024 4,623 7 View

Crispr knockout validation?

I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...

30 March 2024 4,408 2 View

Crispr knockcout validation?

I am doing crispr knockout in T cells. After transfection, I am trying to validate knockout by western blot and sequencing. with western blot, I can see truncated protein in knockout cells whereas...

29 March 2024 5,969 1 View

Is energy minimisation necessary step before molecular docking for protein-ligand interaction?

I have to perform molecular docking on 2 ligands (A structure-absent in literature and B structure-sourced from PubChem) therefore, is energy minimisation necessary for both?

14 March 2024 7,560 3 View

Can anyone provide suggestions for anesthesia in post-natal/ juvenile mice (P10-P16)?

I want to do stereotactic surgery/ microinjection in post-natal/juvenile mice within the age range of P10-P16. I am specifically looking for suggestions on anesthesia. The pup does not get...

10 March 2024 7,785 2 View

How to determine the range of measurement positions one need to set for studying the light scattering intensity in an non-ergodic medium?

Hi, so I have been reading a bunch of research papers on how to do dynamic light scattering experiments in an non-ergodic medium. 1. Couple of them suggest to take the reading from a single...

07 December 2023 3,763 9 View

How do I insert recombinant plasmid into algal cells?

I have synthesized recombinant plasmid with my gene of interest. I need to insert the recombinant plasmid into algal cells. Please suggest experimentation literature for the same.

30 November 2023 9,380 4 View

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Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

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Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Please explain how the plastic input value should be considered from the true stress-strain curve for the bilinear elastoplastic material model ?

I am working on Abaqus/Explicit(Quasistatic ) for the deformation of the auxetic structure model. Please explain how the plastic input value should be considered from the true stress-strain curve...

05 August 2024 454 3 View

"A Markov-like Model for Patient Progression"?

A Markov-like Model for Patient Progression" Markov Chain Monte Carlo (MCMC) Markov Chain Monte Carlo (MCMC) is a powerful computational technique used to draw samples from a probability...

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How to report results of Generalised Linear Mixed Models in a journal article?

Hi everyone, If you have written or come across any papers where Generalised Linear Mixed Models are used to examine intervention (e.g., in mental health) efficacy, could you please share the...

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How to isolate lymphocytes from mouse spleen?

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How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

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