Hi everyone, what is the doubling time of CaCo-2 cells-24 or 48 h? Also, I would be greatful for the ideal media composition (% of FCS and NEAA). Thank you!
Hello, I have a somewhat weird question: has anyone had problems with campylobacter growing in the second half of the strip GLU through ERO? Have you managed to correct it? There is some...
19 July 2024 3,094 2 View
which publication do you recommend for submitting an article on the philosophical and sociological analysis of AI vs Society
30 January 2024 3,918 2 View
preprints on various scientific topics
04 July 2023 5,965 3 View
Hi, I am looking for a company operating in the field of artificial meat. By operating, I mean production or advanced stage of pre-production. I am looking for because we have some good solutions...
24 November 2022 7,258 8 View
I have seen in the literature that short chain poly-lactic acid (polylactide; PLA) oligomers are water soluble. But I am unable to find a commercial source of this type of oligomers. Does anyone...
31 October 2022 1,419 6 View
Can anyone suggest a mercury-free LED light source for fluorescence microscopy? I'm looking for the simplest and most economical replacement for mercury bulbs. Not the complicated stuff with many...
05 October 2022 6,057 3 View
Please only answer if you have done this and do not send me paper links. Thanks. Hi technical Gurus :) So, I am working on a cytotoxicity assay based on the Roche's WST-1 reagent. Every setup is...
15 September 2022 1,611 0 View
For various reasons, I need to avoid DMSO with my cells. So I wonder if isopropyl alcohol (2-propanol) could be used instead of DMSO to create my stock solutions library? I have learned that the...
04 July 2022 3,655 1 View
Based on the literature analysis it could be concluded that one of the most common composition of synthetic lipids to prepare liposome mixture or model membrane (particularly in the work with...
28 June 2022 9,817 3 View
Hello to all I observed a strange phenomenon with CHO-K1 following the change of serum batch (it is surely the toxicity of this batch but I am very curious about the possible nature of this...
21 April 2022 6,157 3 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
Are there any fluorescently labeled anti-Alpaca secondary antibodies raised in Donkey? So far I have only been able to find anti-Alpaca secondaries raised in Goat. Or is this not possible due to...
04 August 2024 4,255 1 View
Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...
04 August 2024 8,894 1 View
Hello. I am working on ROS production of two systems: system A is cerium oxide and hydrogen peroxide, system B is cerium oxide nanoparticle, hydrogen peroxide and potassium bromide. I did some...
04 August 2024 5,974 3 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View