Is there any software/code for studying the effects of wind on vegetation?

I'm searching a (free) software that simulates the effect of strong wind on the vulnerability a tree.

15 December 2020 8,119 3 View

Do you know a reference on action of Heisenberg-Weyl algebra on some basis states?

I am looking for references on Heisenberg-Weyl algebras, namely I am interested in the action of algebra elements on some basis states, i.e. matrix elements in a certain basis.

12 November 2020 181 3 View

Should I share my Doctoral dissertation with a Creative Common License?

Disseminating your PhD dissertation. I am working on publishing activity from my PhD thesis, for which I have carried out three different qualitative investigations. I am extrapolating case...

30 September 2020 7,119 4 View

Does anyone use/have/try any antibody anti OVGP1 in oviduct cells/tissue?

I am looking for an antibody to show the expression of OVGP1 in porcine oviduct cells in culture. Is there one from Sigma and others form ABCAM. But an antibody is too expensive (at least for us,...

31 March 2020 7,273 2 View

Will e-beam deposited cobalt etch in HCl?

Hi, I have a sample of e-beam deposited cobalt that I dipped in 1:1 HCl:H2O at room temperature. The cobalt did not etch. Cobalt is supposedly easily etched with most acids, so I am confused why...

12 March 2020 6,301 3 View

How do I make a rarefaction curve for multiple sample simultaneously in R?

I just tried out the rarecurve function in R for one of my samples, and it worked perfectly- however, I have 72 samples to include in the curve, but I can't find a way to run them all in the code...

17 August 2019 7,938 1 View

Contact mechanics of UHMWPE, better approach ?

I would like know some differences between the contact mechanics of the thin films vs bulk materials. What is the better contact mechanics to evaluate UHMWPE with a thickness of 1 mm in a...

14 January 2019 8,001 2 View

Blunt cloning using Klenow fragment, what is going wrong?

Hi everyone, I am currently trying to clone a fragment (~1700bp) cut with ECORI into a vector (~5300 bp) linearized by ECORV. Since ECORI leaves cohesive ends I am using Klenow Fragment...

07 December 2018 9,865 5 View

How would you study trust in the blockchain context?

I am particularly interested in how blockchain might allow for new forms of trust in the digital environment and beyond. One way of doing this, I reckon, is to find accessible use cases and study...

27 March 2018 1,033 4 View

Can sodium azide be autoclaved?

27 October 2017 2,811 3 View

Buffer to disrupt platelet alpha granules for PF4 quantification?

Hello! I want to quantify by ELISA the secreted (from platelets poor plasma) and the non-secreted (from platelet lysate) PF4 before and after TRAP stimulation. I will use the ELISA from R&D...

03 March 2021 1,499 2 View

Buffer Exchange using column packed with sephadex G-25 effect on protein concentration?

Hello, If i am doing a buffer exchange for an antibody of 1mg/mL, does the elution lose protein in the process of buffer exchange? For example, if i flow through 500 uL of 1mg/mL sample, and...

03 March 2021 6,299 3 View

How optimize the Size Exclusion Chromatography?

Hello! I'll do a size exclusion chromatography, but I only have an open column, and I'll perform the peptide extraction from yeast, using buffer lysis (sodium phosphate 50 mM/NaCl 30 mM/DNAse and...

01 March 2021 2,215 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

What do I do with DLS peaks that are from the buffer?

I'm looking at the aggregation of my protein sample using DLS. Unfortunately, my buffer (20mM HEPES) also results in a set of peaks. These are at approximately 1 and 1000 d.nm. The lowest peak...

01 March 2021 9,015 2 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

What is the most suitable server for predicting protein structures in bacteria?

I have used the i-Tasser several times, however it has been unavailable for several days. I tried the swiss-model, but the output was not very pleasant due to the model used. Is there any other...

28 February 2021 4,521 3 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View