Damaris Lorenzo

4 Questions 2 Answers 0 Followers

Questions related from Damaris Lorenzo

Does the SUMO protease need to inactive after de digestion?

Hi everyone, I have to use the SUMO protease to cleave the SUMO protein from my recombinant protein. After that I will put in contact (ice, one hour) the product of the digestion with the resin...

12 June 2019 6,743 1 View

Can amicon ultra-15 centrifugal filter centrifuge at 4 ºC?

Hi! I need to centrifuge using amicon ultra-15 to concentrate my protein. I have some doubts. First of all, if I can do it at 4 ºC, if it would be better for proteins, although in the protocol it...

15 May 2019 9,670 4 View

BCS stock solution

I have a technical question, I need to know how to prepare a BCS stock solution, is it soluble in water? To prepare the stock solution, need I buy the compost as a Bathocuproinedisulfonic acid...

01 January 1970 6,324 1 View

More virulence mutant strain than the wild type strain

Which could be the reason for a more virulence mutant strain than the wild type strain? The mutant strain lost a gene related to copper detoxification. And the survival of mutant strain resulted...

01 January 1970 7,865 4 View