In most of the cases during Tissue culture the pH of the media is suitably adjusted prior to autoclaving. What impact does autoclaving have on pH? Does it increase or decrease and why? What impact does it have on the overall tissue culture procedure?
Thanking you.
The pH changes during autoclaving a bit (it drops a bit).
Why? ===> If you need a certain pH after autoclaving you need to work a bit on it (adding some NaOH before autoclaving)
The pH changes during autoclaving a bit (it drops a bit).
Why? ===> If you need a certain pH after autoclaving you need to work a bit on it (adding some NaOH before autoclaving)
The pH before and after autoclaving is different. It generally falls 0.3 to 0.5 after autoclaving. Nutrient medium pH ranges 5-6 is suitable for growth of plants. pH higher than 6.0 makes the nutrient medium hard and less than 5 doesn't allow satisfactory gelling of agar gel. for further knowledge read this paper.
http://link.springer.com/article/10.1007%2FBF00269825
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