I want to knock out gene by cas9 protein in RAW 264.7 cell, One of the essential factors is to transfected enough cas9 protein and gRNA complex (RNP) to the cell, thus electrophoresis may be a good choice, but the problem is that I don`t know whether the nucleofector kit of Lonza( which is used to transfect plasmid or siRNA to RAW 264.7 cell, cat# VCA-1003) is OK for protein transfection, the instruction didn`t mentioned that it is capable for protein transfection, Thus I want to know does anyone use this the same kit to transfection protein, does it can transfect to the nucleic of the cell, whether it can cut the gene efficiently. If not, what other methods your use to transfect with protein.
That should work. See publication by Lonza: Efficient CRISPR/Cas9 Delivery Using
Nucleofector™ Technology: Comparison of
Plasmid- and RNP-based Editing or independent protocols, see e. g. https://www.protocols.io/view/cas9-rnp-nucleofection-for-cell-lines-using-lonza-hd8b29w
Uwe Borgmeyer Hi Uwe, Thank you so much. the reference is just what I search for, and I will try it recently.
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