Shijie Fan

3 Questions 10 Answers 0 Followers

Questions related from Shijie Fan

How to overexpress PD-L1 in HEK293T to do immunoprecipitation?

I want to overexpress PD-L1 in HEK293 to do immunoprecipitation. I designed a pcDNA3.1 within N-terminal 6xHis full-length PD-L1 and transfected it into HEK293T by PEI. I used RIPA to get 293...

04 April 2023 6,361 4 View

Is reduced p62 level contradictory to accumulation of LC3B-I?

We used WB to analysis tumor sample from Hepa1-6 transplanted to C57BL6J mouse flank. In drug treatment group, we observed reduced p62 level and accumulation of LC3B-I. We assumed that reduced...

15 March 2021 1,803 3 View

Why I got dim and diffused LC3B bands in WB?

I used strong RIPA to make samples from mouse liver, then adjusted protein concentrations by BCA. Finally I used 2x loading buffer and boiled samples. I runned 12%, 13.5%, 15% gels, 90v ->...

23 July 2019 6,458 11 View