What is a primitive enzyme?

Dear valued researchers, I came across a term "primitive enzyme". I did my reading but still I do not understand much about the enzyme. From my understanding, the primitive enzyme exert broader...

04 May 2018 1,477 0 View

Is it appropriate to simulate the properties of mutated protein on molecular and protein level using PepDraw and ProtParam respectively?

Dear valued researchers, I am working on a protein consisted of 461 amino acids. My colleagues and I are focusing on a 14 amino acids-based well-conserved motif. We have developed a model (E.coli)...

03 April 2018 3,962 0 View

Other than trypan blue exclusion method, is there any cell viability detection assay that is not depending on the mitochondrial metabolism?

My work is using a toxicant that has been known to impair mitochondrial function, hence I am looking for a detection method that able to quantitatively identify viable cell without relying on the...

01 February 2016 3,053 7 View

Why is the alignment sequence between COBALT and UniProt different?

Dear valued researchers,I am running protein sequence alignment using COBALT. However, I have encountered a conflict of information when I tried using UniProt Align software. The arrangement and...

03 April 2015 5,872 9 View

What is the main criteria (physical) that can differentiate between inducible and non-inducible enzymes?

31 December 2014 8,787 3 View

What solvent can I used for Paal Knorr reaction between 6-amino hexanoic acid and 2.5 hexanedione?

I am try to make the Paal Knorr reaction between 2.5 hexnedione and 6-amino hexanoic acid, my problem is type of solvent to use, because 6-hexaminohexanoic acid is soluble in water but I am not...

02 March 2021 4,443 2 View

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02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View

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28 February 2021 9,936 2 View

When is it unnecessary to change organisational culture?

As a former management practitioner, I recall a generally accepted view that changing organisational culture is difficult, so you should only attempt it if the organisational change that you are...

27 February 2021 2,595 8 View