Hisyam Abdul Hamid

6 Questions 4 Answers 0 Followers

Questions related from Hisyam Abdul Hamid

What is a primitive enzyme?

Dear valued researchers, I came across a term "primitive enzyme". I did my reading but still I do not understand much about the enzyme. From my understanding, the primitive enzyme exert broader...

05 May 2018 1,483 0 View

Is it appropriate to simulate the properties of mutated protein on molecular and protein level using PepDraw and ProtParam respectively?

Dear valued researchers, I am working on a protein consisted of 461 amino acids. My colleagues and I are focusing on a 14 amino acids-based well-conserved motif. We have developed a model (E.coli)...

04 April 2018 3,970 0 View

Does temperature affect trichloroacetic acid fixation?

Hi everyone! I am currently performing sulforhodamine B assay to assess cell viability following protocol published by Vichai and Kirtikara (2006) using HEK293T cells. In this protocol, the...

08 August 2017 2,732 1 View

Other than trypan blue exclusion method, is there any cell viability detection assay that is not depending on the mitochondrial metabolism?

My work is using a toxicant that has been known to impair mitochondrial function, hence I am looking for a detection method that able to quantitatively identify viable cell without relying on the...

02 February 2016 3,060 7 View

Why is the alignment sequence between COBALT and UniProt different?

Dear valued researchers,I am running protein sequence alignment using COBALT. However, I have encountered a conflict of information when I tried using UniProt Align software. The arrangement and...

04 April 2015 5,878 9 View

What is the main criteria (physical) that can differentiate between inducible and non-inducible enzymes?

Greetings, Is it possible to manipulate the non-inducible enzymes to become inducible? Just curious.

01 January 2015 8,792 3 View