Does someone know the protocol for extraction of sperm membrane proteins for SDS page?

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For Rt PCR?

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Is it OK to anneal GaN HEMT epitax after some AlGaN recess. what are its pros and cons?

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Mohammad Mehdi Ommati

HI Dear Yaqoob Bhat,

Recently one of my friends with name of Dr Zilong Sun from Shanxi agricultural university, Shanxi, China, wrote an article in this area, but this manuscript is unpublished now, then I cant send you his method, unfortunately, you can contact him for this method, if you couldn't find him, please tell me. I will call him for you and after his allowance, I will send you immediately.

But you can search about this topic if you are in hurry:

After extraction of proteins by his method, protein samples will be loaded on the 12% SDS-PAGE gel followed by a 12 h trypsin digestion at 37°C (trypsin: protein=1:20).

Regards,

Mehdi

Yaqoob Bhat

Mohd Mehdi

sir i couldnt find or contact him...please sir can you help further