11 January 2024 1 4K Report

I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?

More Ganapati Bhat's questions See All
How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Hello fellow researcher .. What is the meaning of your name?

I would like to create a small guide containing the meanings of the names .. Thanks to everyone who cooperates with me in achieving it.

03 March 2021 5,266 4 View

Is There Any Feasible Method To Test Fluorescent Compounds Other Than Fluorescence Spectrometers ??

Is There Any Feasible Method To Test The Efficiency Of Fluorescent Compounds Other Than UV Spectrometers ? Suggestions Would Be Appreciated !

02 March 2021 5,785 3 View

Is it possible to do piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch?

How to do Piezoelectric coupling in ANSYS-APDL or COMSOL between tapered substrate and rectangular piezoelectric patch? Piezoelectric coupling between the rectangular substrate and rectangular...

02 March 2021 9,833 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Adherance of Polyaniline coating over stainless steel?

Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...

01 March 2021 8,220 2 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Why is the sum of the elements in a row in a Q matrix equal to zero?

When explaining substitution models, the substitutions are expressed as Q matrix. Why is the sum of the elements in a row zero?

28 February 2021 3,864 3 View

How to model the battery pack in PSIM or PSCAD?

Hello, I have simulated a single battery cell using Thevenin equivalent circuit. For a battery pack, I have a large number of cells connected in series and parallel connections. Is there any way...

28 February 2021 2,116 1 View

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Extraction of viral DNA from human blood?

Hi, I am looking to detect CMV DNA in immunocompromised leukemia patients followed by subsequent end-point PCR. Can anyone suggests a suitable kit for extraction of CMV DNA from human blood...

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From my gene of interest, Sox9, when cut into my recipient plasmid, pEZT-BM, this may include two WPRE regions and CMV promoter. This would take place if I were to cut with KpnI and NotI. As such,...

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CMV- PCR versus IgM ELISA for studying co-infection ?

We have been looking for the fueling effects of cytomegalovirus on HIVpositive patients. patients serum was tested for CMV by PCR and ELISA for IgM. We found discrepant results, that IgM positives...

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A bovine oocyte-specific transcription factor cannot overexpress in mammalian cells?

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What is causing the delay in expression of EGFP in HEK293T transient transfection with pAAV plasmid?

I have an AAV2 backbone and I am trying to create viruses for my further experiments. I am making an AAV CMV EGFP virus as a negative control and it is giving me a problem. The EGFP expression in...

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Why when I generate a stable cell line I get a mixed phenotype even after a single cell selection?

Hi all, I have generated a few cell lines and I keep getting this mixed problem. I transfect the cells with 50-500ng of the plasmid (CMV promoter and SV40 for the selevtion marker). I add...

11 December 2019 3,522 3 View

Will a non-poxviral genome that is placed under a poxviral promoter and between two pox viral genes be expressed by CMV promoter of pCIneo plasmid?

If I construct a recombinant plasmid in the following order of genes in pCIneo plasmid: Fowlpox virus gene 1- early late promoter of poxviral origin - Non-poxviral gene - Fowlpox virus gene...

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What is the best method to detect simultaneously VZV, JEV, CMV and EBV virus by single PCR assay and gel electrophoresis?

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10 November 2019 5,435 1 View

Which mammalian promotor is suitable for weak but steady expression via transduction in mammalian cells?

I want to transduce mammalian cells with lentiviral system and later inoculate transduced cell into mice but I don't want to highly overexpress my protein after transferring the cells to mice but...

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