11 January 2024 1 4K Report

I have plant sample with mixed infection of CMV and potyvirus, is there any way I can separate CMV only?

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Can we mark 'EFL Learners shifting from general digital to AI technologies' as technological transition?

After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...

08 August 2024 8,964 4 View

How to generate a citation of my paper from ResearchGate?

How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...

08 August 2024 6,688 3 View

Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,618 1 View

How to fix background error in rietveld refinement of one XRD peak using GSAS-II?

I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II

05 August 2024 5,288 2 View

Why might the impedance values for DI water and 0.1X PBS buffer solution exhibit a decreasing and increasing trend, respectively over time (HP 4194A)?

Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...

05 August 2024 3,781 2 View

How can I add own Henry coefficients in Aspen Plus?

Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?

05 August 2024 2,331 2 View

Can usage of AI tools like chat GPT in research work is recommendable ?

AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.

05 August 2024 1,842 3 View

Usage of internal standards in LC-MS/MS analysis?

Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...

05 August 2024 3,082 6 View

ANY free software for reconstructing neurons in the microscopic image?

Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...

04 August 2024 4,725 2 View

How effective is the Citi Bloc standard basket in enhancing the accuracy and comparability of international construction cost assessments?

Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...

04 August 2024 8,996 1 View

Similar questions and discussions
Why the fibroblasts transfected with puromycin plasmids are dying after treatment of puromycin?

Hello, everyone. I would like ask one question. I transfected fibroblasts with EF1A-myod-puro using lipofectamine 3000. Promoter for puro is CMV. After transfection 48 hours, I did puromycin...

23 June 2024 4,902 3 View

Low-yield of Cas9 plasmid after maxi-preparation. Anyone has the same issues?

Hi~ We are making different Cas9 plasmids such as regular spCas9, base editing and prime editing under different promoter such as CMV and CAG. During selection of colonies, we always get a good...

22 May 2024 7,335 0 View

Hi. Can I use a plasmid with CMV promoter in cell-free in vitro system?

I need to express some peptides in cell-free system (fe TnT) but my plasmid has CMV promoter (no T7 or Sp6).

21 May 2024 4,963 4 View

Can I add growth medium to virus-infected cell culture plates?

Hello all. I grew some cells for virus infection. I infected the cells with CMV (Cytomegalovirus) 8 days ago. The cells have not started lysis yet, but the plate is about to get dry. I wonder if I...

08 May 2024 7,051 3 View

What could be the reason for no expression of protein after transfection in HEK293 cells?

I have cloned my genes in a CMV vector and transfected them in HEK293 cells. After harvesting the cells, the expression level at the transcriptional level is increased compared to the control...

23 December 2023 2,506 10 View

Why CMV promoter and SV40 promoter is used used in mammalian expression vectors?

I am having a doubt regarding CMV promoter and SV40 promoter in mammalian expression vector 1. Why CMV and SV40 promoters are used in mammalian expression vectors why not others? 2. There is a CMV...

05 December 2023 1,830 3 View

CMV and EF-1alpha promoter in same plasmid?

Hi I was wondering whether CMV and EF-1alpha promoters can be put in a single plasmid? Are there any compatibility issues between the two promoters? Thank you.

31 October 2023 9,918 2 View

Why is Cytomegalovirus not routinely tested among blood donors in several countries like Nigeria?

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06 September 2023 4,740 1 View

Why is transfection efficiency low when using Lipofectami™ 3000 Transfection Reagent with WT Hella cells for transient transfection?

I used 0.5 ug plasmid. Cell confluency around 60%. Transfection time is 48 hour. I used empty plasmid (pCMV-3Tag-4A) and only Lipofectamine as a negative control. However, I used EGFP plasmid tag...

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What are the predominant viruses those affecting garlic crops and their impact on crop yield loss?

Which specific species and genera of potyviruses, carlaviruses, and allexiviruses significantly impact the quality and quantity of garlic yields on a large-scale production level?"

12 July 2023 529 3 View

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