Can anybody suggest troubleshooting for development of positive control in DNA fragmentation assay?

I have been trying to develop DNA fragmentation by hydrogen peroxide (1mM Concentration). till now i have tried conventional, spin column and DMSO-TE-SDS methods for DNA isolation. But i am not...

11 December 2014 244 3 View

I would like to analyse apoptosis by flow cytometry technique. Can anybody suggest the dye which can be utilized for this purpose?

I am having Propidium iodide, Acridine orange, ethidium bromide, bis benzimide. Which can be best out of this? I have read various papers which made me more confused about this. Can anybody help...

10 November 2014 6,624 24 View

What can be done to reduce the edge effect in cell culture multiplate (96 well plate)??

08 September 2014 6,305 7 View

Is it worth doing an NMR of a plant fraction?

Nowadays many researchers do NMR of plant fractions, but does it give any useful information? I am having active plant fraction. Should I go for NMR? Is it worth doing it?

10 November 2013 9,466 8 View

How to use HP20 resin for fractionation?

I want to further fractionate my water fraction for isolating active molecule.

09 October 2013 9,503 11 View

My column cracked when I change my mobile phase solvent from non polar to nonpolar:polar solvent. Can anybody tell me the solution for this?

Column cracking due to trasition of non polar to polar solvent...

08 September 2013 1,880 16 View

Why does in vitro DNA protection assay or a fragmentation assay require plasmid? Can we use genomic DNA for the same purpose?

DNA damage assay of plant extracts

06 July 2013 2,901 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

How can we obtain a modification factor for creep parameters?

I want to obtain standard creep parameters of salt rock by creep parameters of rectangular samples. Is there a modification factor to convert creep parameters of rectangular (prismatic) rock salt...

01 March 2021 7,131 1 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Anyone found any research papers on solar furnace design calculations (for solar concentration)?

I am thinking about working on solar furnace with heliostats that concentrate sunlight and heat molten salt, but currently no luck with research. Not a lot of papers based on the subject. (check...

01 March 2021 5,023 5 View

Why am i not getting solid after reacting APTES with salicylaldehyde?

The reaction of APTES with salicylaldehyde in ethanol leads to the formation of imine compound yellow solid which is mention in literature, but I am not getting yellow solid I don't know why? when...

28 February 2021 2,955 2 View

Which transition metal salt precursor is best for doping? Why?

I would like to synthesize a transition metal doped TiO2 via solgel method. Hence, I would like to know which metal salt precursor to choose among Chloride, Nitrate, and Sulphate. A clear...

27 February 2021 4,285 3 View

How to compare 2 samples recruited with different sampling methods?

If I want to compare characteristics of 2 groups of patients, one group is recruited from a random sample of the general public while another group is from a sample with a fixed gender (male to...

26 February 2021 3,526 3 View