Piyush Chudasama

8 Questions 33 Answers 0 Followers

Questions related from Piyush Chudasama

Can anybody suggest troubleshooting for development of positive control in DNA fragmentation assay?

I have been trying to develop DNA fragmentation by hydrogen peroxide (1mM Concentration). till now i have tried conventional, spin column and DMSO-TE-SDS methods for DNA isolation. But i am not...

12 December 2014 321 3 View

I would like to analyse apoptosis by flow cytometry technique. Can anybody suggest the dye which can be utilized for this purpose?

I am having Propidium iodide, Acridine orange, ethidium bromide, bis benzimide. Which can be best out of this? I have read various papers which made me more confused about this. Can anybody help...

11 November 2014 6,701 24 View

What can be done to reduce the edge effect in cell culture multiplate (96 well plate)??

I am constantly getting variation in the data set. Need some suggestions if anybody can share their experience.

09 September 2014 6,409 7 View

Does someone know the composition of the preWash solution concentrate provided in a kit for genomic DNA isolation (Spin column based)?

Many kit makers provide the wash solution for removing protein and other divalent ions from the spin.

09 September 2014 5,799 3 View

Is it worth doing an NMR of a plant fraction?

Nowadays many researchers do NMR of plant fractions, but does it give any useful information? I am having active plant fraction. Should I go for NMR? Is it worth doing it?

11 November 2013 9,554 8 View

How to use HP20 resin for fractionation?

I want to further fractionate my water fraction for isolating active molecule.

10 October 2013 9,581 11 View

My column cracked when I change my mobile phase solvent from non polar to nonpolar:polar solvent. Can anybody tell me the solution for this?

Column cracking due to trasition of non polar to polar solvent...

09 September 2013 1,961 16 View

Why does in vitro DNA protection assay or a fragmentation assay require plasmid? Can we use genomic DNA for the same purpose?

DNA damage assay of plant extracts

07 July 2013 2,976 2 View