Can anybody suggest troubleshooting for development of positive control in DNA fragmentation assay?

I have been trying to develop DNA fragmentation by hydrogen peroxide (1mM Concentration). till now i have tried conventional, spin column and DMSO-TE-SDS methods for DNA isolation. But i am not...

11 December 2014 244 3 View

I would like to analyse apoptosis by flow cytometry technique. Can anybody suggest the dye which can be utilized for this purpose?

I am having Propidium iodide, Acridine orange, ethidium bromide, bis benzimide. Which can be best out of this? I have read various papers which made me more confused about this. Can anybody help...

10 November 2014 6,624 24 View

What can be done to reduce the edge effect in cell culture multiplate (96 well plate)??

08 September 2014 6,305 7 View

Does someone know the composition of the preWash solution concentrate provided in a kit for genomic DNA isolation (Spin column based)?

Many kit makers provide the wash solution for removing protein and other divalent ions from the spin.

08 September 2014 5,681 3 View

Is it worth doing an NMR of a plant fraction?

Nowadays many researchers do NMR of plant fractions, but does it give any useful information? I am having active plant fraction. Should I go for NMR? Is it worth doing it?

10 November 2013 9,466 8 View

How to use HP20 resin for fractionation?

I want to further fractionate my water fraction for isolating active molecule.

09 October 2013 9,503 11 View

My column cracked when I change my mobile phase solvent from non polar to nonpolar:polar solvent. Can anybody tell me the solution for this?

Column cracking due to trasition of non polar to polar solvent...

08 September 2013 1,880 16 View

How can plant phytochemistry be included in discussions about whole plant traits?

I am using the concept of plant functional traits (Cornellisen et al., 2003) in a paper. Considering descriptions for whole plant traits (life history; life form; growth form; plant height;...

24 February 2021 6,831 5 View

What is the chemical composition of Cucumis melo roots?

I have found an old pharmaceutical research from 1833 in which emetic power of Cucumis melo roots was confirmed. I cannot find data about chemical constituents of the Cucumis melo roots. Their...

30 October 2020 3,633 3 View

How do you extract a specific chemical from a plant?

I know how to get the general extract from a plant - I can get the total phenols, alkaloids, Flavanoids, etc, but I want to isolate just one particular phenolic compound from the total extract....

21 October 2020 6,384 19 View

What is the best method and solvent for isolation of alkaloids from leaves?

my target to isolate alkaloids from plant leaves, as phytochemistry of plant reported for some alkaloid derivatives , so please guide me.

01 August 2020 7,376 15 View

Phytochemical : looking for Useful chemicals :List of some common and cheapest Chemical Reagent and instrument are mandatory for small Analysis Lab?

07 March 2020 8,329 2 View

How to isolate/quantify a fungal enzyme for comparison of control vs. treatment?

I want to compare the amount of an enzyme's expression (laccase for example) or presence in the tissue as a result of the experimental treatment. Is there a protein similar to an antibody that...

31 December 2019 1,359 2 View

Can anyone suggest the name or contact details of commercial laboratories offering chemical analysis of organic materials through LC-MS/MS?

I have plant methanol, ethyl acetate, n-hexane, and butanol extracts. I am interested in the chemical profiling of each extract through LC-MS/MS. If someone knows the details of commercial labs...

31 December 2019 1,508 4 View

Free or Low-cost Journal with High Impact Factor?

Hi, I want to publish a paper on phytochemistry, and I can't afford something like Elseveir Phytochemistry as it costs $3300+ taxes. So, please help me find a phytochemistry journal with high...

09 October 2019 9,421 3 View

How to patent a new protocole as US.patent ?

I have an open question that is not often asked, as a biochemist I'm working on phytochemistry field and have from time to time problems to solve, sometimes trying to solve a problem may lead to...

30 September 2019 6,382 4 View

Except literature search, how to know a chemical compound belong to which family?

I am writing a review of the herbal plants which requires to elaborate the phytochemistry information. I just wonder if there is any easier way to know which chemical compound belongs to which...

24 September 2019 7,868 3 View