DNA damage assay of plant extracts
Yeah sure, you can use calf thymus DNA also. The purpose of using plasmid is, it is very vulnerable to oxidative stress in Fenton system. So, we can clearly distinguish the linearized plasmid and circular plasmids very easily. So.
Thank you Rajesh.
I have been trying to develop DNA fragmentation by hydrogen peroxide (1mM Concentration). till now i have tried conventional, spin column and DMSO-TE-SDS methods for DNA isolation. But i am not...
11 December 2014 364 3 View
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10 November 2014 6,742 24 View
I am constantly getting variation in the data set. Need some suggestions if anybody can share their experience.
08 September 2014 6,589 7 View
Many kit makers provide the wash solution for removing protein and other divalent ions from the spin.
08 September 2014 5,844 3 View
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10 November 2013 9,594 8 View
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Column cracking due to trasition of non polar to polar solvent...
08 September 2013 2,030 16 View
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06 June 2024 5,489 5 View
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02 April 2024 9,783 3 View
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02 November 2023 5,915 3 View
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02 March 2023 5,520 2 View
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03 December 2022 2,735 2 View
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27 November 2022 9,789 3 View
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18 October 2022 8,302 15 View
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24 February 2021 7,213 5 View
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30 October 2020 3,867 3 View
I know how to get the general extract from a plant - I can get the total phenols, alkaloids, Flavanoids, etc, but I want to isolate just one particular phenolic compound from the total extract....
21 October 2020 6,645 19 View