Did anyone perform CD107a degranulation assay without the use of inhibitors like monensin?

I am planning to perform a degranulation assay using CD107a antibody. Some resources suggest to use inhibitors like monensin to inhibit the intracellular traficking and acidification of the...

21 February 2021 6,260 3 View

How many ug should I choose as the protein amount when doing western blot in liver tissue?

25 January 2021 794 3 View

How to Remove articles from my name?

There are numerous articles connected to my name. I am not the author. I am not going to take the time to individually remove each one.

03 October 2020 6,701 3 View

Any idea about focal adhesion lenght measurement by automatically?

Hi , I have encountered a problem with the measurement of focal adhesion length. I need some advice from you. As measuring all FAs in an individual cell takes so long time by hand I am looking...

03 March 2020 7,239 2 View

Why my chromogenic dye's being spread??

Hello I have a problem as you can see on the photo. The pvdf membrane was stained with chromogenic dye and washed with ddh2o2. I'm sure alcohol isn't mixed into the dh2o2. I used the blotting...

25 February 2019 2,462 2 View

What is the formula for heat exchange between mass oil in a tank and ambient?

I have a tank of lube oil which is not insulated and top of tank is a compartment, in other words, tank is the bottom of the auxiliary compartment. The volume of tank is 25.000 liters. Because we...

27 March 2018 1,388 5 View

Site Directed Mutagenesis, PCR works but no colonies after transformation?

23 February 2016 4,552 4 View

What would happen if we used 45C instead of 65C as annealing temperature of colony pcr?

26 August 2014 179 3 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

What kind of fluid could I use for a pitfall trap that also does not invalidate molecular testing?

Hi, I want to start testing pitfall trap to obtain ants samples, but I need to conduct molecular analysis on those insects. So, what kind of fluid can I use? Ethanol expires too early and I need...

03 March 2021 5,978 5 View

No title

02 March 2021 3,060 3 View

How to repeat an operation on an atom selection for multiple times in PyMol?

Hi, I am trying to construct a multi-layer fibril structure from a single layer in PyMol by translating the layer along the fibril axis. For now, I am able to use the Translate command in PyMol...

02 March 2021 4,569 4 View