I am currently working on some ex vivo viral infection models with treatment with different agents. I found some pieces of information in the literature about pre-treatment with LPS to "strengthen" PBMC's (their viability) and cytokine release.
I usually seed cells on the plate, let them 16h of "rest", and then exposed them to the therapeutic agents (many configurations). I was wondering where to "put" that pretreatment with LPS and for how long should the cells be treated? I saw that some researchers add it before the treatment (which I assumed would be ok for my application), but some of them do it shortly after the treatment.
My idea was:
1. Thawing the PBMC's, adding LPS to the Falcone, leave for "rest day".
2. Wash the cells and seed them.
3. Treatment as usual.
What do you think about that? I have serious doubts if the cytokine profile will make sense after that combination.
Thank you in advance!
Good inquiry,
First, LPS activate NO production as well as some Interleukins.
Regarding your question, from my experience, I dilute LPS in the same therapeutic medium and add them at the same time.
But you should be careful when you read the cytokine expression, each cytokine has its specific treatment period.
Abdalsalam Kmail Thank you very much for your reply, I drew similar conclusions in the course of the further review, so your statement confirmed my assumptions.
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