Didem Baran

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Questions related from Didem Baran

Does somebody use Polyethylenimine (PEI) for RFL6 cell line transfection?

I use nucleofection (Amaxa kit) method for a transfection of RFL6 cells. It' s highly efficient but I would like to try Polyethylenimine (PEI) method for RFL6 cells because it is a relatively...

19 October 2016 367 0 View

Site Directed Mutagenesis, PCR works but no colonies after transformation?

I have been trying to do a site directed mutagenesis for single aminoacide change, using QuickChange II Site Directed Mutagenesis Kit. I have designed my primers using QuickChange Tool. The Tm of...

24 February 2016 4,666 4 View