Does ompA peripelasmic secretion signal cleave specifically and completely from the N-terminal of recombinant proteins expressed in E. coli?

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Huanting Liu

If your target protein accumulated quickly, there will be some non-processed ones left (or harvested before being processed). Slow down your expression and optimising the amino acid at P1' and P2' positions can help.

Amir Maghsoudi

Thanks Liu,

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OmpA signal peptide leads to heterogenous secretion of B. subtilis chitosanase enzyme from E. coli expression system