Amir Maghsoudi

3 Questions 4 Answers 0 Followers

Questions related from Amir Maghsoudi

How would a phage infected E. coli culture look under light microscope, with Gram staining?

do the bacteria lyse completely or do they form aggregates?

05 May 2018 9,138 1 View

No expression in E. coli fed-batch culture on M9 media, but good expression on M9 in erlene-meyer flask?

we get excellent expression on LB and M9 in flask, but no expression in the fed-batch cultures in bioreactor. we have an E. coli bl21(DE3) transformed with pet-24a

09 September 2017 6,332 6 View

Does ompA peripelasmic secretion signal cleave specifically and completely from the N-terminal of recombinant proteins expressed in E. coli?

We're expressing recombinant hGH in E. coli using the ompA peripelasmic secretion signal for soluble expression. From our SDS-PAGE and SE-HPLC results we really doubt if the ompA signal peptide is...

08 August 2017 8,601 2 View