I want to know whether LPS+ATP combination induces inflammasome activation in HUVEC or endothelial cells.
Thanks
A somewhat similar question is asked here:
https://www.researchgate.net/post/Is_it_possible_to_activate_the_NLRP3_inflammasome_through_LPS_and_ATP_in_endothelial_cells
I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?
01 March 2021 9,383 1 View
I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...
15 February 2020 2,245 4 View
We are currently working on a cytosolic protein; however, we think that this protein also has a secreted form and can be detected in the bloodstream. As there is no ELISA kit available for this...
01 February 2020 3,798 2 View
I want to study whether two proteins: Myc-tagged protein A and Flag-tagged protein B, interacts with each other or not. For that I co-expressed these proteins in HEK293T cells, and later (after 48...
27 February 2019 4,191 3 View
I want to determine whether protein A and protein B interacts with each other by co-immunoprecipitation analysis. For that I prepared Myc-tagged protein A and Flag-tagged protein B plasmid DNAs. I...
25 February 2019 9,305 3 View
I have been working on two proteins. And I think that these two proteins interact with each other. To prove that, I analyzed there association by endogenous IP, but unfortunately I could not get...
23 December 2018 5,160 3 View
I know that DAPI binds with DNA in the nucleus and produces fluorescence. But, there are many papers in which they just measure and compare transcription factors, e.g. FOXO1 within different...
20 July 2016 2,299 1 View
I want to induce MRP4 in different cell lines by using some specific inducing agents. Does anybody know about any specific inducing agents for MRP4?
21 January 2015 1,622 1 View
Currently, I am performing luciferase assay to measure promoter activity of a SNP. I have two construct, one contains GG genotype and other contains AA genotype. I am using PGL3 basic as a...
05 January 2015 7,488 7 View
I am going to use real time PCR using Taqman assay. I have experience of RT-PCR by using SYBR but I have no idea about Taqman. Can anyone suggest a protocol for Taqman?
08 December 2014 7,332 6 View
I am using the ATP Assay Kit (Colorimetric/Fluorometric) (ab83355) for measuring intracellular ATP from treated cell lines. Although my standards are wokring fine when using the fluorometric...
15 February 2021 3,746 4 View
Dear Collegues, If we want to perform immunohistochemistry /immunofluorence staining from FFPE human biopsy (vein) samples and study the presence of LPS and or common pathogen like Porphyromonas...
01 December 2020 5,744 2 View
Hello, Has anyone had luck measuring LPS from human plasma? Which ELISa kit do you recommend? I am getting a lot of interference from color of the plasma. Thanks
15 November 2020 6,599 1 View
Hello! I'm starting my Master's project soon, and I'm in the optimization/practice step. I'm stimulating RAW264.7 cells (in a 24 well) with LPS for 18h. I did a first experiment today: a...
21 October 2020 2,761 8 View
In what time do a protein body consumes an ATP molecule for an active process to happen?
02 October 2020 8,135 4 View
I need to test the stimulation production of TNF alpha in the RAW cell line by LPS. I am looking for an easy and cheap experiment to quantify the serial dilution stimulation in this macrophage...
27 September 2020 5,366 6 View
I suspect that under phosphorus deficiency the electron transport chain in the plasmamembrane and mitochondria of soil bacteria and AM fungi, respectively, is disrupted as a result of lower ATP...
30 August 2020 4,659 3 View
Im working with a S. typhimurium strain. Until now when i wanted to have a look at the amount of Lipid A present in the cells, i did silverstainings with proteinaseK treated whole cell samples....
26 August 2020 9,455 1 View
I'm replacing primary neutrophils with the neutrophil-like cell line HL-60 for immunological studies. Do HL-60 cells, differentiated towards the granulocyte phenotype with retinoic acid or DMSO,...
07 June 2020 2,219 3 View
We aim to publish ta high impact journals (If 6+). Compounds showed incredible activities on cancer cell line (submicromolar on several types). I need your recommendation on how to determine...
24 May 2020 1,230 3 View