How to perform cycloheximide chase assay for long half-life proteins (>36 h)?

20 January 2023 0 201 Report

I'm using a cycloheximide chase assay to look into the stability of a protein. Despite giving the cells cycloheximide (100 uM) over a number of time periods (0, 12h, 24h, and 36h), I was unable to detect a drop in protein levels. I cannot use longer time points than 36 h because it is toxic for the cells. Does anybody have suggestions?

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