Does anyone know the diameter (size) of mouse heart endothelial cell?

09 July 2023 1 1K Report

Hi! On average, the diameter of endothelial cells can range from approximately 10 to 20 micrometers. However, there may be variations depending on experimental conditions

Badges
Science topic

More Sailesh Palikhe's questions See All

Does anybody know how many endothelial cells are present in the heart of an adult mouse?

23 July 2023 8,201 1 View

Does LPS+ATP combination induces inflammasome activation in HUVEC?

I want to know whether LPS+ATP combination induces inflammasome activation in HUVEC or endothelial cells. Thanks

08 March 2023 5,173 1 View

How to perform cycloheximide chase assay for long half-life proteins (>36 h)?

I'm using a cycloheximide chase assay to look into the stability of a protein. Despite giving the cells cycloheximide (100 uM) over a number of time periods (0, 12h, 24h, and 36h), I was unable to...

19 January 2023 234 0 View

Etchant for additive manufactured CuCrZr alloys?

Greetings everyone I am trying to etch some additively manufactured CuCrZr alloys but i am not able to get the melt pool can anyone give me some suggestions I have tried with FeCl3 HCL...

16 January 2023 4,409 4 View

Anyone can explain me how non equiatomic high entropy braze foils are made?

I need to understand the processing route of non-equiatomic high entropy braze foils

18 September 2022 9,198 0 View

How can I make stacked bar graph with scatter plot?

I want to make a stacked bar graph with showing each data value as dots. I tried with graph pad prism, version 6, but could not succeed. Does anyone have tried before making such graphs?

01 March 2021 9,899 1 View

What could be the reason for reduced intensity of HE staining of villi?

I performed HE staining of intestinal section of wild type (WT) and Knockout mice (KO). I found that the staining intensity is reduced in case of KO mice. There was no difference in villi length...

20 July 2020 1,442 0 View

Can I collect quantitative data from one sample and qualitative data from another sample on the same research topic and call it a mixed-method?

I am working on a study to understand tourists poor behaviour towards beach cleanliness. For that I am collecting quantitative data. As part of the research, I am also willing to understand the...

15 July 2020 7,494 15 View

How to prevent getting fragmented villi structure in HE staining of small intestine sections?

I am trying to perform HE staining of small intestine. I used OCT solution to make sections. After HE staining when I look my slides under microscope, I do not get intact villi. In much of the...

15 February 2020 2,456 4 View

Is there any method to measure serum protein besides ELISA?

We are currently working on a cytosolic protein; however, we think that this protein also has a secreted form and can be detected in the bloodstream. As there is no ELISA kit available for this...

01 February 2020 3,997 2 View

Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

Mouse CD3 antibody sequence?

Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much

08 August 2024 9,157 4 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

Can I multiplex a mouse monoclonal and rat primary for IHC (mouse brain tissue)?

Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...

06 August 2024 9,710 1 View

How much total RNA concentration to be extracted from sorted plasma cells from bone marrow of C57BL/6 mice for RT-PCR ?

i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...

05 August 2024 8,835 1 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to use Density Functional Theory to calculate carrier mobilities of solid system?

Hello, everyone. I have tried to determine carrier motilities of some materials, by Density Functional Theory, using Quantum ESPRESSO. There are a few methods to do it, like a package called...

04 August 2024 8,894 1 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

In what part of the brain´s rabbit bdnf is located and include references please?

I´ve been unable to find specific information about this neurotrophin in the CNS of rabbits exclusively. There is extensive info in mice, fish and rats, but in brain´s rabbit is hard to find....

04 August 2024 762 1 View