Do Lipofectamine 2000 transfected plasmids get integrated into chromosomes? And if not then how many generations does it remain inside the transfected cell? If transient then how transient?
The stability of the transfection is mainly dependent on the plasmid. Lipofectamine 2000 is a really good transfection reagent that can warranty you a high transfection efficiency.
The transfection agent is "only" responsible for bringing your plasmid or RNA into the cell. Inside it depends on the plasmid itself.
For some cell lines other transfection reagents than Lipofectamine work better (meaning higher efficiency and/or less dead cells), but this needs to be tested.
Transiant or stable Transfection Not relevant to method or material of transfection. Plasmid containing genes for resistance to antibiotics applied if you like G418 , hygromycin , zeocin Is within 1 to 2 months after antibiotic Force you can take stable transfectant..
Lipofectamine 2000 is a very efficient transfection agent, the stability of your knockdown or your over-expression is only related to the half life of your siRNA, plamid in the cell.
beside lipofectamin transfection dependent on cell line and target gene ,If you chek gene expression in unigene(EST) and based on select cell line that accept your target gene efficiently
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