Hey,
maybe some expert can help me. I did qPCR analysis of MEFs that showed severe differentiation defects and thus usually do not differentiate into adipocytes upon treatment with insulin containing medium (ADM). However, I observed a significantly increased expression of IGF-1 for those treated with ADM. My idea was that IGF-1 expression is a direct response to the insulin in the medium due to their similar structure and their capability to bind each other's receptor. Unfortunately I couldn't find any publications proving this.
Is there any known evidence that insulin directly enhances expression of IGF-1 in MEFs? And can someone suggest any publications for further information about this?
Thanks in advance. :)
Insulin stimulates the type I receptor, and our work, we used a conc. of 50 ng/mL to effect the insulin receptor, but not trigger the IGF receptor.
Insulin is required for differentiation of fibroblasts cells. In our studies we compared with the cells treated with and with out insulin. IGF expression was noted.
hello Sven,
you can see this: Murphy LJ, Ghahary A, Chakrabarti S. Insulin regulation of IGF-I expression in rat aorta. Diabetes. 1990;39:657–662.
and or : atvb.ahajournals.org/content/24/3/435.full.pdf
good luck!
To test IGF1 activy in complete culture medium, my suggestion would be to use long-IGF1
Insulin indeed affects the levels of IGF1R and also the expression of the IGf system
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