Divide after collapsing replicates in DESeq?

Hello everyone, currently, I'm trying to run a differential gene expression analysis on an RNA-Seq data set using R and the DESeq2 package. However, there are several technical replicates in the...

06 July 2016 7,285 4 View

Which statistical test suits best?

Hello everyone, I'm currently trying to find out which statistical test I can use to show differences between two or more populations. I had cells, which I infected with a virus +/- additional...

04 May 2016 4,497 6 View

What is energetically more favorable?

Hey, I've got a question which might be very simple. I was asked which interaction of amino acids is more energetically favorable. My problem begins with the what the definition of "energetically...

01 February 2016 7,555 6 View

Does insulin in the medium influence expression of IGF-1 in MEFs?

Hey, maybe some expert can help me. I did qPCR analysis of MEFs that showed severe differentiation defects and thus usually do not differentiate into adipocytes upon treatment with insulin...

02 March 2015 7,353 5 View

What is the 2^-Ct for in qPCR analysis?

Hey, I'm currently writing my bachelors thesis and I was wondering why you do the "2^-Ct" step before normalising the to the reference. All i have found in the internet are methods setting two...

02 March 2015 7,219 3 View

Which statistical test fits best the bisulfite sequencing of DNA from cells I treated with a demethylating substance at different concentrations?

Hey, I did bisulfite sequencing of DNA from cells I treated with a demethylating substance at different concentrations before. I now want to show that the difference between control, negative...

01 February 2015 7,229 9 View

How do you calculate annealing temperature for bisulfite primer?

31 December 2014 5,305 2 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Calculating volume of DNA required from DNA concentration?

Hi I am a bit confused. They are asking me to find out the volume of DNA required in ul (a total of 30-100 ng for genomic DNA) from the DNA concentration in the nanodrop reading which was 404.8...

26 February 2021 5,029 2 View

Has anyone used the Illumina ITS1 primer sets?

Hi everyone, Illumina provides a list of primers to amplify with high taxonomic coverage the ITS1 region for further fungal sequencing, but I cannot find the exact amount necessary of each...

25 February 2021 6,969 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration fors?

Warm greetings My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology (on Human papillomavirus). At this stage, I have almost completed a clinical sample Cervical swabs)...

25 February 2021 1,660 3 View

Is there any one who can provide a donation of HPV detection and genotyping kits in exchange collaboration forms?

Warm greetings! My name is Ayichew S. and I am doing my Ph.D. in Medical Microbiology focusing on Human papillomavirus). At this stage, I have almost completed a clinical sample (Cervical swabs)...

25 February 2021 3,612 3 View

What urea buffer can i use for biotagged protein extraction from streptavidin beads?

Hi all, I have been doing research on biotagged LDB1 proteins in Mel cells. I purify the proteins using M280 streptavidin beads. The yield is very low so I've been trying to optimize it by...

24 February 2021 5,749 3 View

Errors in DNA sequences?

I am trying to better understand the scope of DNA replication and sequencing errors, e.g. 1. I have seen similar error rates of 10e4 to 10e5 for cell & instrumental DNA replication,...

24 February 2021 4,397 3 View