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Hello everyone, currently, I'm trying to run a differential gene expression analysis on an RNA-Seq data set using R and the DESeq2 package. However, there are several technical replicates in the...
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Hey, I'm currently writing my bachelors thesis and I was wondering why you do the "2^-Ct" step before normalising the to the reference. All i have found in the internet are methods setting two...
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Hey, maybe some expert can help me. I did qPCR analysis of MEFs that showed severe differentiation defects and thus usually do not differentiate into adipocytes upon treatment with insulin...
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Hey, I did bisulfite sequencing of DNA from cells I treated with a demethylating substance at different concentrations before. I now want to show that the difference between control, negative...
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Hey! Has anyone made experience with DNA extraction form cells after fixation in formalin? I want to do an Oil-Red O staining of adipocytes and afterwards a DNA extraction. Is there someone who...
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Hey guys, I'm trying to do bisulfite sequencing and therefore I designed some primers using MethPrimer. The Tm ist around 59.5 °C for both forward and reverse. Have you experienced a way which...
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