Divide after collapsing replicates in DESeq?

Hello everyone, currently, I'm trying to run a differential gene expression analysis on an RNA-Seq data set using R and the DESeq2 package. However, there are several technical replicates in the...

06 July 2016 7,285 4 View

Which statistical test suits best?

Hello everyone, I'm currently trying to find out which statistical test I can use to show differences between two or more populations. I had cells, which I infected with a virus +/- additional...

04 May 2016 4,497 6 View

What is energetically more favorable?

Hey, I've got a question which might be very simple. I was asked which interaction of amino acids is more energetically favorable. My problem begins with the what the definition of "energetically...

01 February 2016 7,555 6 View

Does insulin in the medium influence expression of IGF-1 in MEFs?

Hey, maybe some expert can help me. I did qPCR analysis of MEFs that showed severe differentiation defects and thus usually do not differentiate into adipocytes upon treatment with insulin...

02 March 2015 7,353 5 View

anyone familiar with DNA Extraction from Formalin-fixed Cells?

Hey! Has anyone made experience with DNA extraction form cells after fixation in formalin? I want to do an Oil-Red O staining of adipocytes and afterwards a DNA extraction. Is there someone who...

01 February 2015 8,173 3 View

Which statistical test fits best the bisulfite sequencing of DNA from cells I treated with a demethylating substance at different concentrations?

Hey, I did bisulfite sequencing of DNA from cells I treated with a demethylating substance at different concentrations before. I now want to show that the difference between control, negative...

01 February 2015 7,229 9 View

How do you calculate annealing temperature for bisulfite primer?

31 December 2014 5,305 2 View

What is your perception on using visual analysis or quantitative approaches to evaluate data from single-case research designs (n of 1 trials)?

Results of single-case research designs (i.e., n-of-1 trials) are often evaluated by visually inspecting the time-series graph and computing quantitative indices. A question our research team is...

03 March 2021 687 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Do you know good and user-friendly programs to measure plant quantitative traits?

For my research i will need to measure plant quantitative traits (especially leaves area and roots length, but would be nice to add some more information). I recently discovered...

01 March 2021 5,035 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

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Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View